植物遗传资源学报2026，Vol.27Issue(5)：1044-1055,12.DOI:10.13430/j.cnki.jpgr.20260104004
谷子HD-ZIP基因家族鉴定分析及SiHD05的抗旱性验证
Identification and Analysis of the HD-ZIP Gene Family in Foxtail Millet and Drought Resistance Verification of SiHD05
摘要
Abstract
Homeodomain-leucine zipper(HD-ZIP)transcription factors are widely distributed in plants and play crucial roles in regulating plant growth,development,and responses to abiotic stresses.However,systematic identification and functional characterization of this gene family in foxtail millet(Setaria italica L.)remain unclear.In our previous study,we identified the drought-resistant candidate gene Seita.1G253700.1.With the publicly available genome database of foxtail millet variety Yugu 1 combining with bioinformatics analysis,a systematic identification of the HD-ZIP gene family in foxtail millet is conducted,and its physicochemical properties,phylogenetic relationships,conserved motifs,gene structure,chromosomal location and cis-acting elements are analyzed.The results showed that a total of 29 HD-ZIP family members were identified in foxtail millet,with amino acid lengths ranging from 220 to 422 aa,relative molecular weights ranging from 23.99 kDa to 44.41 kDa,and theoretical isoelectric points from 4.60 to 9.63.All members were predicted to be localized in the nucleus by subcellular localization analysis.According to the phylogenetic tree,the HD-ZIP family members of foxtail millet were distributed in the HD-ZIP Ⅰ and HD-ZIP Ⅱ subfamilies,while no members were found in the HD-ZIP Ⅲ and HD-ZIP Ⅳ subfamilies,and significant differences in motif types and gene structures were observed among members of different subfamilies.The 29 genes were distributed on 7 chromosomes,with chromosome 9 having the largest number of genes,and the number of genes was not proportional to chromosome length.cis-acting element analysis revealed that the HD-ZIP gene family contains multiple abiotic stress-responsive elements,including abscisic acid-responsive elements and drought-inducible MYB binding sites.After transferring the SiHD05 gene into Arabidopsis thaliana,comprehensive analysis of phenotypes and physiological indices showed that overexpression of SiHD05 exacerbated leaf wilting under drought stress and significantly increased the accumulation of superoxide anion and abscisic acid,confirming that this gene negatively regulates drought resistance in Arabidopsis.This study provides a basic data for exploring the mechanism of the homeodomain-leucine zipper SiHD05 in foxtail millet and for research aimed at improving crop stress resistance.关键词
HD-ZIP/谷子/基因家族/生物信息学/SiHD05/抗旱Key words
HD-ZIP/Setaria italica L./gene family/bioinformatics/SiHD05/drought resistance引用本文复制引用
侯昌晔,侯绪妍,唐帆,吴志英,周文镜,张慧媛,崔雯雯,崔颖,严加坤..谷子HD-ZIP基因家族鉴定分析及SiHD05的抗旱性验证[J].植物遗传资源学报,2026,27(5):1044-1055,12.基金项目
国家自然科学基金项目(32460540,32301315) （32460540,32301315）
陕西省创新能力支撑计划(2024CX-GXPT-03,2024-CXY-093) （2024CX-GXPT-03,2024-CXY-093）
河南省科技攻关项目(252102110159) （252102110159）
河南省现代农业产业体系建设专项(HARS-22-04-Z2)National Natural Science Foundation of China(32460540,32301315) （HARS-22-04-Z2）
Shaanxi Provincial Innovation Capability Support Program(2024CX-GXPT-03,2024-CXY-093) （2024CX-GXPT-03,2024-CXY-093）
Henan Provincial Key Scientific and Technological Research Project(252102110159) （252102110159）
Special Fund for Modern Agricultural Industrial System Construction of Henan Province(HARS-22-04-Z2) （HARS-22-04-Z2）
