植物研究2026，Vol.46Issue(3)：411-424,14.DOI:10.7525/j.issn.1673-5102.2026.03.003
白桦BpLUXs基因鉴定与功能验证
Identification and Preliminary Functional Analysis of the BpLUXs Gene in Betula platyphylla Suk.
摘要
Abstract
Photoperiod serves as the key environmental cue governing seasonal growth and growth cessation in forest trees.Within the perception and transduction of photoperiodic signals,LUX ARRHYTHMO(LUX),a core component of the circadian clock Evening Complex(EC),plays a pivotal role.In the present study,a total of 47 BpLUX homologous genes were identified through genome-wide analysis in the reference genome of Betula platyphylla.Bioinformatic analyses revealed that BpLUX1,BpLUX5,and BpLUX18 harbor typical Evening Element(EE)cis-acting elements.All three genes possessed characteristic MYB conserved domains,while BpLUX1 and BpLUX5 additionally exhibited typical circadian rhythmic expression patterns.Using CRISPR/Cas9-mediated genome editing,loss-of-function mutants for BpLUX1(bplux1)were successfully generated,whereas targeted knockout of BpLUX5 proved unsuccessful.Phenotypic characterization demonstrated that under short-day(SD)conditions,wild-type birch ceased growth almost entirely by day 19 in response to shortened photoperiods,whereas bplux1 mutants remained actively growing.Furthermore,while no significant difference in plant height was observed in wild-type plants,bplux1 mutants displayed significantly increased height,exhibiting a delayed growth cessation phenotype.These findings indicate that BpLUX1 is a key gene mediating short-day responses and growth cessation in birch.Transcriptome analysis further revealed that under SD conditions,eleven differentially expressed genes(DEGs)were down-regulated in bplux1 mutants,including BpRCAR1(Regulatory Component of ABA Receptor 1)involved in ABA(abscisic acid)signaling,whereas seven DEGs were up-regulated,including BpRGL1(RGA-Like1)associated with GA(gibberellin)signaling.Notably,potential binding sites between BpLUX1 and key DEGs in ABA and GA signaling pathways were identified,suggesting that BpLUX1 may influence growth cessation through modulation of phytohormone signaling.Collectively,this study provides a theoretical basis for understanding the role of BpLUX1 in photoperiod-regulated growth cessation in birch.关键词
白桦/BpLUX/光周期/生长停止Key words
Betula platyphylla/BpLUX/photoperiod/growth cessation分类
农业科技引用本文复制引用
潘梦婷,刘月影,郑志民..白桦BpLUXs基因鉴定与功能验证[J].植物研究,2026,46(3):411-424,14.基金项目
国家自然科学基金青年科学基金项目(32401540) （32401540）
中国博士后科学基金第75批面上项目(2024M750382). （2024M750382）
