植物研究2026，Vol.46Issue(3)：505-516,12.DOI:10.7525/j.issn.1673-5102.2026.03.011
适度干旱对党参叶和根多糖合成差异调控
Differential Regulation of Polysaccharide Synthesis in Leaves and Roots of Codonopsis pilosula under Moderate Drought Stress
摘要
Abstract
By investigating metabolite levels,enzyme activities,and gene expression associated with polysaccharide synthesis in the leaves and roots of Codonopsis pilosula under moderate drought conditions,this study amied to reveal the differential regulatory mechanisms governing polysaccharide synthesis in the tissues.During the flowering period,plants were subjected to two treatments:normal moisture(CK,0%PEG-6000)and moderate drought(DH,15%PEG-6000).Samples were collected at 7,14,and 21 days post-treatment.Using ultraviolet spectrophotometry,high-performance liquid chromatography(HPLC),and high-throughput sequencing,we quantified the levels of metabolites involved in the polysaccharide synthesis pathway,enzyme activities,and gene expression in root and leaf tissues of C.pilosula.Results indicated that under moderate drought conditions,the levels of upstream metabolites—sucrose,fructose,and glucose—differred between leaves and roots.Specifically,the proportion of glucose increased in roots,whereas it decreased in leaves.Enzyme activities in the polysaccharide synthesis pathway also exhibited distinct tissue-specific responses:sucrase activity increased in both tissues,while the activities of monosaccharide conversion-related enzymes(galE,GAE,UXS1,and UER1)increased in leaves but decreased in roots.Moreover,significant changes in sucrase activity occurred earlier in leaves than in roots.Gene expression analysis further revealed tissue-specific differences in the regulation of polysaccharide synthesis.Among the 10 sucrose invertase regulatory genes,2 were upregulated and 3 downregulated in leaves,whereas 6 were upregulated and 1 downregulated in roots.For the 14 genes encoding monosaccharide conversion enzymes,7 were upregulated and 2 downregulated in leaves,compared to 2 upregulated and 5 downregulated in roots.Pearson correlation analysis showed that under moderate drought,the number of genes positively correlated with enzyme activity significantly increased in both tissues.Specifically,gene expression in leaves was positively correlated with monosaccharide conversion enzyme activity,whereas gene expression in roots was positively correlated with sucrose transferase activity.Collectively,moderate drought differentially regulated polysaccharide synthesis in C.pilosula leaves and roots by modulating upstream metabolite levels,sucrose transferase activity,and monosaccharide conversion-related gene expression,thereby controlling tissue-specific polysaccharide synthesis and accumulation.关键词
党参/适度干旱/多糖组分/酶活性/差异基因表达Key words
Codonopsis pilosula/moderate drought/CPPs component/enzymatic activity/differential genes expression分类
农业科技引用本文复制引用
管廷蕾,陈增,黄储,孙美乐,陈红刚,杜弢,王惠珍..适度干旱对党参叶和根多糖合成差异调控[J].植物研究,2026,46(3):505-516,12.基金项目
国家自然科学基金地区科学基金(81760683) （81760683）
甘肃省科技计划(22JR11RA111) （22JR11RA111）
国家中药材产业技术体系建设专项资金(CARS-21). （CARS-21）
