植物研究2026，Vol.46Issue(3)：517-530,14.DOI:10.7525/j.issn.1673-5102.2026.03.012
夏蜡梅属植物花香成分测试与聚类分析
Testing and Clustering Analysis of Floral Scent Compounds of Calycanthus
摘要
Abstract
To investigate the chemical composition and diversity of floral scent volatiles in plants of the genus Calycanthus,the floral scent compounds of nine Calycanthus species or varieties were analyzed using a combination of headspace solid-phase microextraction(HS-SPME)and static headspace(HS)coupled with gas chromatography-mass spectrometry(GC-MS).Principal component analysis(PCA)and cluster analysis were employed to compare and evaluate the diversity of volatile compounds in the flowers of Calycanthus plants.The results showed that the numbers of volatile compounds detected in the flowers of C.chinensis,C.floridus and C.floridus'Athens'were 16,21,and 23(by SPME)and 5,21,and 23(HS),respectively.The volatile profile of C.chinensis was dominated by alkanes(HS-SPME)or terpenes(HS),while that of C.floridus consisted mainly of esters,especially ethyl acetate.The volatile compositions of C.floridus'Athens'were more diverse,including ethyl acetate,ethyl isobutyrate,ethyl butyrate and ethyl isobutyrate.Among the hybrid varieties,except for'Meixia'which was rich in terpenes,the others contained relatively high levels of esters.The order of ester content from high to low was'Hongyun','Venus','Hongxiao','Fencan','Xiaodie'and'Meixia'.PCA revealed that the volatile compounds of C.chinensis were the most distinct from those of the other Calycanthus plants,whereas the hybrid varieties showed greater similarity to the female parents(C.floridus and C.floridus'Athens').Cluster analysis of the volatile compounds obtained by the two methods indicated that the static headspace approach corresponded more closely to the genetic relationship among the tested Calycanthus plants.Specifically,hybrids from the same cross combination('Fencan','Xiaodie','Meixia'and'Hongyun')clustered together with their parents C.chinensis and C.floridus,while they were separated from C.floridus'Athens'and its hybrid offspring('Hongxiao'and'Venus').Based on the floral volatile composition results,C.floridus and C.floridus'Athens'were rich in ester volatile compounds,suggesting their potential for development of fragrance chemicals and applications in aromatic gardens.关键词
夏蜡梅属/花香成分/顶空固相微萃取/静态顶空/气质联用/杂交品种Key words
Calycanthus/floral scent compounds/HS-SPME/HS/GC-MS/hybrid variety分类
农业科技引用本文复制引用
陈香波,刘方舟,冯宜冰..夏蜡梅属植物花香成分测试与聚类分析[J].植物研究,2026,46(3):517-530,14.基金项目
国家林业和草原局生物安全与遗传资源管理专项(KJZXSA202308) （KJZXSA202308）
上海市绿化和市容管理局科研专项(G220202). （G220202）
