中国现代中药2026，Vol.28Issue(5)：991-1001,11.DOI:10.13313/j.issn.1673-4890.20251128002
基于高通量测序的8种易霉变中药饮片的表面细菌多样性分析
Surface Bacterial Diversity in Eight Mildew-prone Decoction Pieces Based on High-throughput Sequencing
摘要
Abstract
Objective:To investigate the bacterial community structure and species diversity on the surfaces of eight types of mildew-prone decoction pieces,to analyze differences in microbial communities among decoction pieces from different sources,and to assess the potential risk of pathogenic bacteria.Methods:Moldy regions of eight decoction pieces,including Platycladi Semen,Vespae Nidus,Trichosanthis Fructus,Arecae Pericarpium,Polygalae Radix,Amomi Fructus Rotundus,bran-roasted Myristicae Semen,and processed Morindae Officinalis Radix,were selected.High-throughput sequencing was used to perform amplicon sequencing of the V5-V7 regions of the bacterial 16S rRNA gene.Samples from moldy regions were grouped based on medicinal parts and aflatoxin contamination status.According to medicinal parts,samples were classified into a root group(Polygalae Radix,and processed Morindae Officinalis Radix),a fruit/seed group(bran-roasted Myristicae Semen,Amomi Fructus Rotundus,Trichosanthis Fructus,Platycladi Semen,and Arecae Pericarpium),and an animal-derived group(Vespae Nidus).According to aflatoxin contamination status,samples were divided into an aflatoxin-detected group(Platycladi Semen,Polygalae Radix,Vespae Nidus,and bran-steamed Myristicae Semen)and an aflatoxin-undetected group(Arecae Pericarpium,Trichosanthis Fructus,processed Morindae Officinalis Radix,and Amomi Fructus Rotundus).Bioinformatic analyses and comparisons of community structure were subsequently performed.Results:A total of 177 bacterial species were identified in the moldy regions of the eight decoction pieces.Polygalae Radix and processed Morindae Officinalis Radix contained the highest numbers of unique bacterial species.No unique bacterial species were found in Vespae Nidus or Amomi Fructus Rotundus,while the relative abundance of Clostridium botulinum in Trichosanthis Fructus was approximately 0.2%.Principal coordinate analysis(PCoA)showed that the microbial community structure in moldy regions differed markedly between the root group and the animal-derived group,whereas the differences between the fruit/seed group and the above two groups were relatively small.In addition,there were marked differences between the aflatoxin-detected and aflatoxin-undetected groups,with the latter showing a higher abundance of Bacillus(95.80%).Conclusion:Differences exist in the bacterial community composition of mildew-prone decoction pieces,with greater variation observed in the root and animal-derived groups.Enrichment of Bacillus was observed in the moldy region of Amomi Fructus Rotundus,and a potential pathogenic bacterium(Clostridium botulinum)was detected in moldy Trichosanthis Fructus.These findings suggest that targeted bacterial contamination monitoring strategies should be established for different types of decoction pieces in storage and quality control.关键词
高通量测序技术/易霉变中药饮片/细菌多样性/肉毒杆菌Key words
high-throughput sequencing/mildew-prone decoction pieces/bacterial diversity/Clostridium botulinum分类
医药卫生引用本文复制引用
徐婷婷,隗立国,宋钕帆,徐政安,丹珍卓嘎,白贞芳,任广喜,刘春生..基于高通量测序的8种易霉变中药饮片的表面细菌多样性分析[J].中国现代中药,2026,28(5):991-1001,11.基金项目
国家药品监督管理局委托研究课题(2023-JYB-900202-046) （2023-JYB-900202-046）
