中国现代中药2026，Vol.28Issue(5)：1002-1009,8.DOI:10.13313/j.issn.1673-4890.20260125001
蛤蚧不同炮制品特征图谱的建立及化学模式识别研究
Establishment of Characteristic Fingerprints and Chemical Pattern Recognition for Different Processed Products of Gecko
摘要
Abstract
Objective:To establish the characteristic fingerprints of clam powder-fried Gecko(CPGG),Gecko(cutting),and wine-processed Gecko and compare the quality differences among different processed products of Gecko using chemical pattern recognition technology.Methods:The characteristic fingerprint and reference fingerprint of CPGG were established,and the common peaks in the reference fingerprint were calibrated and identified.The characteristic fingerprints of Gecko(cutting)and wine-processed Gecko were also established.Similarity evaluation,cluster analysis(CA),and orthogonal partial least squares-discriminant analysis(OPLS-DA)were adopted to analyze the characteristic fingerprints of different processed products of Gecko.Results:Seven common peaks were calibrated and identified in the established reference fingerprint of CPGG,including three amino acids,one nucleosides,and three other components.The similarity evaluation showed that the similarities of CPGG,Gecko(cutting),and wine-processed Gecko fingerprints to the reference fingerprint of CPGG were 0.990-0.999,0.964-0.990,and 0.971-0.988,respectively.CA and OPLS-DA could effectively distinguish CPGG from its different processed products,and three key differential markers were identified as inosine(Peak 5),creatinine(Peak 2),and creatinine isomer(Peak 3).Among them,the relative peak area of Peak 5(inosine)in CPGG was higher than that in Gecko(cutting)and wine-processed Gecko,which could serve as a key marker for distinguishing CPGG from the other two processed products.Conclusion:The established characteristic fingerprints combined with chemical pattern recognition can objectively and effectively distinguish CPGG from its different processed products,providing a reference for the quality control and evaluation of CPGG.关键词
蛤粉烫蛤蚧/蛤蚧(切制)/酒蛤蚧/饮片/炮制品/特征图谱Key words
clam powder-fried Gecko/Gecko(cutting)/wine-processed Gecko/decoction pieces/processed product/characteristic fingerprint分类
医药卫生引用本文复制引用
袁桂平,聂舸,张旗,唐堂,万咪容,张春桃,涂远博..蛤蚧不同炮制品特征图谱的建立及化学模式识别研究[J].中国现代中药,2026,28(5):1002-1009,8.基金项目
江西省药品监督管理局科研项目(2022JS16) （2022JS16）
