中国现代中药2026，Vol.28Issue(5)：1020-1030,11.DOI:10.13313/j.issn.1673-4890.20260109004
香贝养荣汤指纹图谱建立和多成分含量测定
Fingerprinting and Multi-component Determination of Xiangbei Yangrong Decoction
摘要
Abstract
Objective:To establish the fingerprint and a multi-component determination method for Xiangbei Yangrong Decoction.Methods:Twelve batches of Xiangbei Yangrong Decoction were prepared.Chromatograms were determined by high performance liquid chromatography(HPLC).The fingerprint was established and the reference fingerprint was generated via the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 edition).Similarity evaluation was performed on the 12 batches of Xiangbei Yangrong Decoction,and the common peaks in the reference fingerprint were identified.The contents of 16 components in Xiangbei Yangrong Decoction were determined by ultra-high performance liquid chromatography-mass spectrometry(UPLC-MS).Results:The similarities between the fingerprints of the 12 batches of Xiangbei Yangrong Decoction and the reference fingerprint were all greater than 0.95.A total of 41 common peaks were assigned,of which 16 common peaks were identified by comparison with reference substances.Peaks 4,10,12,13,15,16,18,19,21,23,31,33,35,36,38,and 39 were identified as rehmannioside D,paeoniflorin,verbascoside,liquiritin,ferulic acid,hesperidin,ginsenoside Rg1,platycodin D,ginsenoside Rb1,glycyrrhizic acid,ligustilide,atractylenolide Ⅱ,atractylenolide Ⅰ,α-cyperone,dehydropachymic acid,and pachymic acid,respectively.Linearity tests showed good linear relationships for all 16 components within their respective ranges.The precision and stability tests showed that the relative standard deviations(RSDs)of the peak areas of the 16 components were all≤2.7%.The repeatability test showed that the RSDs of the contents of the 16 components were all≤3.6%.The spiked recovery test showed that the average spiked recovery rates of the 16 components ranged from 97.2%to 101.9%,with RSDs all≤2.8%.The content of the 16 components in Xiangbei Yangrong Decoction was 92.88-163.10,956.32-1 670.21,186.54-366.93,258.01-376.41,300.36-492.33,2 773.39-4 586.73,182.60-380.93,155.11-297.26,162.99-336.37,421.56-836.43,434.12-944.82,150.46-366.78,194.32-424.47,111.83-297.47,503.65-891.90,and 384.86-891.92 μg·g-1,respectively.Conclusion:The established fingerprint analysis method and content determination method for Xiangbei Yangrong Decoction are simple and reliable,providing a reference for its quality control.关键词
香贝养荣汤/质量控制/高效液相色谱法/超高效液相色谱-质谱法/指纹图谱/多成分含量测定Key words
Xiangbei Yangrong Decoction/quality control/high performance liquid chromatography/ultra-high performance liquid chromatography-mass spectrometry/fingerprint/multi-component determination分类
医药卫生引用本文复制引用
呙霞,胡夏逢,曾伟杰,陈莹..香贝养荣汤指纹图谱建立和多成分含量测定[J].中国现代中药,2026,28(5):1020-1030,11.基金项目
吴阶平医学基金会临床科研专项(320.6750.2022-19-32) （320.6750.2022-19-32）
