遵义医科大学学报2026，Vol.49Issue(5)：472-482,11.
转录组学分析揭示HMGB1在敌草快中毒肾损伤中的作用机制
Investigation of HMGB1-mediated mechanisms in diquat-induced renal injury via transcriptomics
摘要
Abstract
Objective To investigate the molecular mechanisms of high-mobility group box 1 protein(HMGB1)involvement in diquat(DQ)induced acute kidney injury(AKI)using the public database.Methods Differenti-al gene expression data for diquat poisoning(GSE27170)and HMGB1(GSE18721)were analyzed and down-loaded from the GEO database,with GSE4607 serving as the validation set.Differential genes were subjected to KEGG,GO and PPI analyses.The hub genes were screened using R language.Immune infiltration analysis was performed via the Sangerbox website.Finally,animal experiments were conducted to preliminarily validate the relationship between HMGB1 expression and DQ-induced renal injury.Results Fifty-two hub genes closely relat-ed to immune response,oxidative stress,and metabolic processes were identified.Further screening through the MCC algorithm,PPI network,and independent validation dataset verification yielded four hub genes:NQO1,GCLM,SRXN1,and HMOX1.Immune infiltration and correlation analysis of these four hub genes revealed posi-tive correlations with NK cells and B cells,with particularly higher correlations observed for CD4 T cells and MO macrophages.Finally,animal experiments confirmed that HMGB1 expression plays a critical role in the renal pathological injury induced by DQ poisoning.Conclusion NQO1,GCLM,SRXN1,and HMOX1 are hub genes associated with DQ poisoning and HMGB1,participating in DQ induced AKI by regulating oxidative stress,met-abolic disorders,cellular response to toxins,and immune response pathways.关键词
高迁移率组蛋白B1/敌草快中毒/急性肾损伤/转录组学/氧化应激Key words
high mobility group box1 protein/diquat poisoning/acute kidney injury/transcriptomics/oxida-tive stress分类
医药卫生引用本文复制引用
任红林,胡杰,吴瑾,廖娅,张小军,夏彩云,陆元兰..转录组学分析揭示HMGB1在敌草快中毒肾损伤中的作用机制[J].遵义医科大学学报,2026,49(5):472-482,11.基金项目
国家自然科学基金资助项目(NO:82460385) （NO:82460385）
贵州省基础研究项目[NO:黔科合基础-ZK(2023)一般579] （2023）
贵州省基础研究计划项目[NO:黔科合基础-MS(2025)388]. （2025）
