遵义医科大学学报2026，Vol.49Issue(5)：483-492,10.
脾脏X线照射对脓毒症相关急性肝损伤的保护作用
Splenic X-ray irradiation alleviates sepsis-associated acute liver injury
摘要
Abstract
Objective To investigate the protective effect and underlying mechanisms of early lowdose splenic X-ray irradiation(Irra)against sepsisassociated acute liver injury(SALI).Methods The sepsis model was estab-lished by classic cecal ligation and puncture(CLP)to induce sepsis-associated acute liver injury(SALI).At 6 hours postoperation,the spleen was locally irradiated with Xrays(8 Gy).One hundred and twenty male SD rats were randomly assigned to two major cohorts(n=60)for either survival observation or endpoint analysis.Each cohort was further divided into four subgroups(n=15):Sham,Sham+Irra,CLP,and CLP+Irra.The 7day survival rate and spleen index were recorded.Serum ALT,AST and hepatic cytokines(IL6,IL1 β,TNFα)were measured by ELISA.Levels of Fe2+,GSH,and MDA in liver tissues were determined.Histopathological chan-ges(H&E staining),mitochondrial ultrastructure(TEM),macrophage markers CD68 and CD206(ICH),and reactive oxygen species(ROS)were assessed.Protein expression of GPX4,ACSL4,and SLC7A11 was assessed by Western blotting.Results Compared with the CLP group,CLP+Irra significantly improved the 7 day survival rate and liver function,attenuated histopathological and mitochondrial damage,and reduced hepatic levels of the proinflammatory cytokines IL6,IL1β,and TNFα(P＜0.01).Irradiation also promoted the shift of hepatic macrophages from the M1 to the M2 phenotype(P＜0.01),decreased levels of ROS,Fe2+,and MDA,and re-stored GSH content(P＜0.01).Moreover,irradiation upregulated the expression of SLC7A11 and GPX4 while downregulating ACSL4 compared with the CLP group(P＜0.01).Conclusion Splenic Xray irradiation protects against SALI,potentially through a mechanism involving the inhibition of the ferroptosis pathway.关键词
脓毒症相关急性肝损伤/脾脏X线照射/单核巨噬细胞/铁死亡Key words
sepsis-associated acute liver injury/splenic X-ray irradiation/monocyte-macrophage/ferroptosis分类
医药卫生引用本文复制引用
孙田静,马娟,黄晓飞,段海真,喻安永..脾脏X线照射对脓毒症相关急性肝损伤的保护作用[J].遵义医科大学学报,2026,49(5):483-492,10.基金项目
贵州省科技计划项目[NO:黔科合基础MS(2025)387] （2025）
贵州省教育厅青年科技人才成长项目[NO:黔教技(2024)141] （2024）
贵州省卫生健康委科学技术基金资助项目(NO:2025GZWJKJXM0643). （NO:2025GZWJKJXM0643）
