中药与临床2026，Vol.17Issue(3)：1-4,22,5.
HPLC测定不同产地不同批次牡丹皮中芍药苷含量
Determination of paeoniflorin in different batches of Moutan Cortex from different producing areas by HPLC
摘要
Abstract
Objective:To determinate paeoniflorin content in different batches of Moutan Cortex from different origins.Methods:Firstly,an HPLC method for the determination of paeoniflorin in Moutan Cortex was established and validated.A total of 32 batches of Moutan Cortex from authentic producing regions and large-yield producing regions on the market were selected.A Waters Symmetry Shield C18 column(250 mm×4.6 mm,5 μm)was used in this experiment.The mobile phase consisted of ace-tonitrile and 0.1%phosphoric acid solution at a ratio of 16∶84,and the detection wavelength was set at 230 nm.Samples were pre-treated by water reflux extraction for 30 min.The paeoniflorin content was determined,and the differences among samples from different producing regions were analyzed.Results:The average content of paeoniflorin in Moutan Cortex was 0.76%in Tongling,Anhui,0.73%in Bozhou,Anhui,0.67%in Nanling,Anhui,0.75%in Heze,Shandong,0.59%in Luoyang,Henan,0.66%in Dianjiang,Chongqing and 0.63%in Yuncheng,Shanxi.Conclusion:The average paeoniflorin contents of Moutan Cor-tex in Tongling,Bozhou and Heze main production areas are relatively higher.关键词
牡丹皮/芍药苷/不同产地/高效液相色谱法Key words
Moutan Cortex/paeoniflorin/different producing areas/HPLC分类
医药卫生引用本文复制引用
朱力阳,彭伟,周许,吴明权,陈韵蓓..HPLC测定不同产地不同批次牡丹皮中芍药苷含量[J].中药与临床,2026,17(3):1-4,22,5.基金项目
四川省中医药管理局课题(2021MS206,2023MS599) （2021MS206,2023MS599）
成都市医学科研课题(2025088) （2025088）
成都市委校联合创新基金项目(WXLH202502019) （WXLH202502019）
全国中药特色技术传承人才培训项目(国中医药人教函[2023]96号) （国中医药人教函[2023]96号）
