安徽农业大学学报2026，Vol.53Issue(2)：207-215,9.DOI:10.13610/j.cnki.1672-352x.20260514.003
盐驯化通过调节光合作用和抗氧化系统提高大豆幼苗耐盐性
Salt acclimation improves salt tolerance of soybean seedlings by regulating photosynthesis and antioxidant systems
摘要
Abstract
[Objective]This study aimed to investigate the effect of salt acclimation on soybean salt tolerance,elucidate the physiological mechanisms underlying salt acclimation-enhanced salt tolerance in soybean.[Method]After being subjected to 80 mmol·L-1 salt acclimation treatment for 24 h,the plants were then transferred to different salt treatment solutions(0,120,160 and 200 mmol·L-1 NaCl)for cultivation.The effect of salt stress on plant height,photosynthetic properties and antioxidant system of soybean seedlings were investigated.[Result]The plant height and above-ground fresh weight of the control group decreased as the salt treatment concentration increased.The chlo-rophyll index of the leaves in the salt-acclimation group decreased.The chlorophyll index,flavonoid index and multi-color fluorescence parameters of leaves in the salt-acclimation group showed a trend of first increasing and then de-creasing with the increase of salt treatment concentration.In contrast,the chlorophyll index and nitrogen balance index of the control group showed a gradually decreasing trend with the increase of salt treatment concentration.The maxi-mum photosynthetic efficiency of salt-acclimation group did not differ significantly between 120 and 160 mmol·L-1 salt treatment and significantly decreased by 24.17%under 200 mmol·L-1 salt treatment.The maximum photosyn-thetic efficiency of the control group was significantly decreased under 160 and 200 mmol·L-1 salt treatment(de-creasing by 41.49%and 30.13%,respectively).The net photosynthetic rate,transpiration rate,intercellular CO2 con-centration and stomatal conductivity of the salt-acclimation group did not change significantly.Meanwhile,the above-mentioned photosynthetic parameters of the control group decreased with the increase of the salt treatment concentration.Under the same concentration of salt treatment,the above photosynthetic parameters of the salt-acclimation group were significantly higher than that of the control group.The SOD and POD activities of the salt-acclimation group under salt stress were significantly higher than those under 0 mmol·L-1 NaCl conditions.[Conclusion]After salt acclimation,soybean seedlings can maintain a good growth state and relatively stable pho-tosynthetic characteristics under salt stress.The antioxidant enzyme activities enhanced and alleviated the oxidative damage caused by salt stress,ultimately improving the salt tolerance of soybean seedlings.关键词
大豆/盐驯化/耐盐性/多光谱荧光成像/抗氧化活性Key words
soybeans/salt acclimation/salt resistance/multicolor fluorescence imaging/antioxidant activity分类
农业科技引用本文复制引用
周梦杰,尚辉,张晓燕,纪晓伟,吴谷丰,黄璐,陈新,薛晨晨..盐驯化通过调节光合作用和抗氧化系统提高大豆幼苗耐盐性[J].安徽农业大学学报,2026,53(2):207-215,9.基金项目
江苏省沿海开发集团青年创新工作室项目(2023YHDTQC06) （2023YHDTQC06）
江苏省种业振兴揭榜挂帅项目(JBGS-2021-014) （JBGS-2021-014）
