安徽农业大学学报2026，Vol.53Issue(2)：240-248,9.DOI:10.13610/j.cnki.1672-352x.20260107.026
基于拮抗和多基因序列分析平菇种质资源遗传多样性
Genetic diversity of Pleurotus ostreatus germplasm resources based on antagonistic and multigene sequence analysis
摘要
Abstract
[Objective]This study aimed to reveal the genetic variation and phylogenetic relationships of the tested Pleurotus ostreatus strains by combining cross-trial analysis with multi-gene sequence analysis techniques.[Method]Using 15 strains of P.ostreatus as test samples,the antagonistic interactions among strains were first determined through antagonism assays,and then the mycelial growth characteristics of the test strains on PDA plates were observed.Concurrently,genomic DNA was extracted from the test strains,and multiple gene se-quences were amplified to construct a phylogenetic tree.[Result]Mycelial growth characteristics revealed that the growth rates of Jinxiu-2 and Jinxiu-1 exhibited extremely significant differences compared to other strains,reach-ing to 4.722 mm·d⁻1.Xiangxiu-1 showed the slowest growth rate at 2.361 mm·d⁻1.Antagonistic interactions ex-isted to varying degrees among most strains,with only Jinxiu-2 and Jinxiu-1 exhibiting no antagonistic response.The total GC content in the ITS sequences ranged from 43.44%to 46.71%,with genetic distances varying from 0.002 to 0.397.The total GC content in the TEFI-α sequences ranged from 52.06%to 55.06%,with genetic dis-tances ranging from 0.021 to 0.445.Based on the ITS+TEFI-α phylogenetic tree,antagonistic response,and growth rate results,test strains C,D,F,G,N,and P formed one group,strains A,E,H,I,J,L,and M formed an-other group,and strain B stood alone as a separate group.[Conclusion]This study combined antagonism assays with polygenic sequence analysis to demonstrate that P.ostreatus germplasm resources exhibit substantial genetic diversity,with significant genetic differences among strains.These findings provide concrete evidence for the classification,identification,and utilization of P.ostreatus germplasm resources.关键词
平菇/生长特性/拮抗试验/ITS序列/TEFI-α序列/遗传多样性Key words
Pleurotus ostreatus/growth characteristics/antagonistic test/ITS/TEFI-α/genetic diversity分类
农业科技引用本文复制引用
温家敏,吴民深,程飞..基于拮抗和多基因序列分析平菇种质资源遗传多样性[J].安徽农业大学学报,2026,53(2):240-248,9.基金项目
国家自然科学基金项目(32160359) （32160359）
广西特色中草药林下复层栽培技术及加工剩余物利用研究与示范(桂科AD23049001) （桂科AD23049001）
