动物医学进展2026，Vol.47Issue(6)：25-30,6.
碳纳米管修饰氟苯尼考对其抗胞内感染鼠伤寒沙门氏菌活性研究
Study on the Activity of Carbon Nanotubes Modified Florfenicol Against Intracellular Infection of Salmonella typhimurium
摘要
Abstract
To explore the antibacterial activity of carbon nanotube(CNT)nanomaterials adsorbed with flor-fenicol(FF)against intracellular Salmonella typhimurium(ST)infection.Using mouse mononuclear mac-rophage(RAW264.7)as the model cell,a model of intracellular infection of RAW264.7 cells by Salmonel-la typhimurium was constructed.And the conditions such as the multiplicity of infection(MOI),the clear-ance of extracellular bacteria,and the lysis conditions were respectively optimized;In this experiment,the minimum inhibitory concentration values of FF and CNT-FF complexes against ST and the cytotoxicity test were determined to evaluate their anti-intracellular ST effects and biocompatibility.The results showed that the intracellular infection system of Salmonella typhimurium was composed of multiple infections with MOI=200 infecting cells for 1 h.Gentamicin at 50 μg/mL was administered for 0.5 h to kill the extra-cellular bacteria,and the lysis buffer was PBS containing 0.02％Tween-20.The intracellular bacterial clearance test indicated that the clearance rate of intracellular ST in the CNT-modified FF group(66％)was significantly higher than that in the FF group(24％)(P＜0.05);The cytotoxicity tests indicated that when the concentration of the CNT-FF complex was not higher than 30 μg/mL,it had no effect on cell sur-vival rate,showing good biocompatibility.In this study,a model of intracellular infection of mononuclear macrophages by Salmonella typhimurium was successfully constructed.The CNT-FF complex showed strong antibacterial activity against intracellular ST,which can provide a reference basis for formulating a reasonable drug administration plan for the treatment of intracellular ST.关键词
碳纳米管/氟苯尼考/鼠伤寒沙门氏菌/胞内菌Key words
Carbon nanotubes/Florfenicol/Salmonella typhimurium/Intracellular bacteria分类
农业科技引用本文复制引用
王彤,李存,李泽慧,方汉卿,李木子,池佳瑶,李青蔚,常潍帆,李嘉敏,赵思俊..碳纳米管修饰氟苯尼考对其抗胞内感染鼠伤寒沙门氏菌活性研究[J].动物医学进展,2026,47(6):25-30,6.基金项目
中国动物卫生与流行病学中心创新基金项目(2021221200) （2021221200）
