动物医学进展2026，Vol.47Issue(6)：47-54,8.
猫血浆中氟雷拉纳液相色谱-串联质谱检测方法的建立
Establishment of an LC-MS/MS Method for Fluralaner Determination in Cats' Plasma
摘要
Abstract
Fluralaner(Flur)is an isoxazoline insecticide and acaricide characterized by rapid absorption,safety,high efficacy,long-lasting effects,and good tolerability.To investigate the pharmacokinetics of com-pound Flur drops in cats,a liquid chromatography—tandem quadrupole mass spectrometry(LC-MS/MS)method for the detection of Flur in feline plasma was established using Flur-D4 as an internal standard.Acetonitrile was used as the extraction solvent,with water-bath ultrasonic-assisted extraction followed by vacuum evaporation and redissolution prior to LC-MS/MS analysis and quantification via the internal standard method.The results showed that the feline plasma matrix did not interfere with the quantitative detection of Flur,and the method exhibited good specificity;The method exhibited high sensitivity,with a limit of detection(LOD)of 5 ng/mL and a limit of quantification(LOQ)of 10 ng/mL;matrix effects were insignificant.A standard curve was prepared using solvent-based standard solutions,and Flur exhibited a good linear relationship(R2＞0.99)in the concentration range of 10 ng/mL to 6 000 ng/mL.The spiked samples with different concentration levels,the mean accuracy ranged from 92.60％to 116.70％,with both intra-and inter-batch coefficients of variation below 10％,meeting methodological requirements.Spiked samples with different concentration remained stable when stored frozen at-18℃ or below for 6 months or after repeated freeze-thaw cycles;and the processed solution of spiked samples remained stable when stored at 4℃ for 72 hours.The standard stock solution and intermediate standard solution were stable for 9 months,while the working standard solution was stable for 2 months.The results indicate that the estab-lished method exhibits good specificity,high sensitivity,and is stable and reliable,making it suitable for pharmacokinetic studies of Flur drops in cats.关键词
氟雷拉纳/液相色谱-串联质谱/内标法/猫/血浆Key words
Fluralaner/LC-MS/MS/Internal standard/Cat/Plasma分类
农业科技引用本文复制引用
过海天,范丽萍,徐浩,张贺超,杨亚军,李剑勇,周淑贞..猫血浆中氟雷拉纳液相色谱-串联质谱检测方法的建立[J].动物医学进展,2026,47(6):47-54,8.基金项目
佛山市自筹经费类科技创新项目(2220001004511) （2220001004511）
中国农业科技创新工程项目(25-LZIHPS-02) （25-LZIHPS-02）
