分析化学2026，Vol.54Issue(5)：777-786,10.DOI:10.19756/j.issn.0253-3820.261066
具有类氧化酶活性的氧化铁/氮磷掺杂碳纳米材料用于谷胱甘肽的比色检测
Ferric Oxide/Nitrogen-Phosphorus-Doped Carbon Nanomaterials with Oxidase-Mimicking Activity for Colorimetric Detection of Glutathione
摘要
Abstract
Using pyrrole,phytic acid,and ferric chloride as raw materials,the hydrogel was rapidly synthesized via a one-pot method,followed by high-temperature calcination to obtain ferric oxide/nitrogen-phosphorus-doped carbon nanomaterials(Fe2O3/NP-C).The morphology and structure of the as-prepared Fe2O3/NP-C were characterized by scanning electron microscopy(SEM),transmission electron microscopy(TEM),X-ray diffraction(XRD),and X-ray photoelectron spectroscopy(XPS).Fe2O3/NP-C exhibited high oxidase-like activity,enabling efficient catalysis of colorless 3,3′,5,5′-tetramethylbenzidine(TMB)oxidation to blue oxidized TMB(oxTMB).Based on the pronounced inhibitory effect of glutathione(GSH)on this chromogenic system,a highly sensitive and rapid colorimetric sensor for GSH detection was constructed.Under the optimized conditions,the developed sensor showed a linear detection range for GSH from 0.1 μmol/L to 20 μmol/L with a detection limit(3σ)of 57 nmol/L.The results showed that GSH suppressed the reaction through a dual-pathway mechanism,which could both scavenge the superoxide radicals and hydroxyl radicals generated during the catalytic process and directly reduce the oxidation product oxTMB.The sensor exhibited excellent selectivity for GSH and was successfully applied to quantitative determination of GSH in cell lysates.关键词
氧化铁/氮磷掺杂碳纳米材料/类氧化酶/谷胱甘肽/比色分析/传感器Key words
Ferric oxide/nitrogen-phosphorus-doped carbon nanomaterials/Oxidase mimics/Glutathione/Colori-metric analysis/Sensor引用本文复制引用
楼茜,王勇..具有类氧化酶活性的氧化铁/氮磷掺杂碳纳米材料用于谷胱甘肽的比色检测[J].分析化学,2026,54(5):777-786,10.基金项目
国家自然科学基金项目(Nos.32160600,62301242)、赣鄱俊才支持计划—主要学科学术和技术带头人培养项目-领军人才(学术类)(No.20232BCJ22017)和现代农业装备与技术教育部重点实验室开放基金项目(No.MAET202311)资助. Supported by the National Natural Science Foundation of China(Nos.32160600,62301242),the Ganpo Juncai Support Plan-Training Project for Academic and Technical Leaders of Major Disciplines in Jiangxi Province(No.20232BCJ22017)and the Open Funding of the Key Laboratory of Modern Agricultural Equipment and Technology(Jiangsu University),Ministry of Education(No.MAET202311). （Nos.32160600,62301242）
