分析化学2026，Vol.54Issue(5)：787-795,9.DOI:10.19756/j.issn.0253-3820.261080
基于丝素蛋白-铂纳米酶的可穿戴电化学传感贴片用于汗液中尿酸的检测
Wearable Electrochemical Sensing Patch Based on Silk Fibroin-Platinum Nanozyme for Detection of Uric Acid in Sweat
摘要
Abstract
To meet the daily monitoring demand of uric acid(UA)in sweat,a novel wearable electrochemical sensing patch was constructed for highly sensitive detection of UA.The patch integrated platinum nanozyme with uricase-like activity,synthesized in situ using silk fibroin(SF)as a reducing and stabilizing agent,with a flexible laser-induced graphene(LIG)electrode.Leveraging the reducing capability of amino acid residues in SF,platinum nanoparticles were uniformly grown in situ.Modification of the LIG electrode surface with this composite yielded an integrated ″catalytic center-conductive substrate″ architecture,which not only increased the number of active sites but also facilitated the interfacial electron transfer.Electrochemical characterization revealed that the fabricated sensing patch exhibited a distinct UA oxidation peak at approximately 0.4 V.The peak current demonstrated a good linear relationship with UA concentration in the range of 0.1‒200 μmol/L,with a detection limit(S/N=3)as low as 0.03 μmol/L.Furthermore,the patch displayed excellent selectivity for UA,effectively eliminating interference from common coexisting substances such as glucose and urea.In simulated sweat samples,the recovery rates for spiking UA ranged from 101.6%to 104.7%.Critically,when applied to direct detection of UA in authentic human sweat,the patch validated its outstandingin vivodetection performance.This study developed a novel electrochemical sensing strategy based on uricase-like activity of Pt-SF,offering a viable pathway for the non-invasive,highly sensitive analysis of UA in sweat.关键词
丝素蛋白-铂纳米酶/类尿酸氧化酶活性/尿酸/激光诱导石墨烯/电化学传感Key words
Silk fibroin-platinum nanozyme/Uricase-like activity/Uric acid/Laser-induced graphene/Electro-chemical sensing引用本文复制引用
刘宇,柳建林,王炜烨,李峰,谷成成..基于丝素蛋白-铂纳米酶的可穿戴电化学传感贴片用于汗液中尿酸的检测[J].分析化学,2026,54(5):787-795,9.基金项目
国家自然科学基金项目(Nos.22274086,22404091)和山东省自然科学基金项目(No.ZR2023QB003)资助. Supported by the National Natural Science Foundation of China(Nos.22274086,22404091)and the Shandong Provincial Natural Science Foundation(No.ZR2023QB003). （Nos.22274086,22404091）
