分析化学2026，Vol.54Issue(5)：814-824,11.DOI:10.19756/j.issn.0253-3820.251333
炭疽芽孢生物标志物2,6-吡啶二羧酸检测技术的研究进展
Research Progress on Detection Techniques for Anthrax Spore Biomarker Dipicolinic Acid
摘要
Abstract
Anthrax is a severe zoonotic infectious disease caused by Bacillus anthracis,whose spores possess extremely strong infectivity and environmental resistance,posing a major threat to public health and biosecurity.As a unique biomarker of anthrax spores,trace and highly specific detection of 2,6-pyridinedicarboxylic acid(DPA)is crucial for early diagnosis of anthrax,epidemic early warning,and biosecurity prevention and control.In recent years,DPA chemical detection techniques have become a research hotspot in this field due to their advantages of simple operation,low cost,high sensitivity and rapid response.Firstly,this review briefly introduced the research status of biological detection techniques for Bacillus anthracis and its spores,including polymerase chain reaction,loop-mediated isothermal amplification and CRISPR-Cas techniques.Subsequently,it focused on summarizing the latest research progress of DPA detection based on chemical techniques,including principles,advantages,disadvantages,and differences in limit of detection of optical,electrochemical and hyphenated detection techniques.Finally,combined with the current technical bottlenecks,future optimization directions and application prospects of DPA detection technologies were prospected.关键词
炭疽病/2,6-吡啶二羧酸/生物标志物/检测技术/评述Key words
Anthrax/Dipicolinic acid/Biomarker/Detection techniques/Review引用本文复制引用
王鑫,高梅梅,杨婉琪,周金荣,赵世强,陈玮,常春..炭疽芽孢生物标志物2,6-吡啶二羧酸检测技术的研究进展[J].分析化学,2026,54(5):814-824,11.基金项目
国家自然科学基金项目(No.22203079)、河南省重点研发与推广项目(科技攻关)(No.252102310047)和河南省研究生教育改革与质量提升工程项目(No.YJS2024JD04)资助. Supported by the National Natural Science Foundation of China(No.22203079),the Key Research and Development Program of Henan Province(Science and Technology Project)(No.252102310047)and the Graduate Education Reform and Quality Improvement Project of Henan Province(No.YJS2024JD04). （No.22203079）
