分析化学2026，Vol.54Issue(5)：892-900,9.DOI:10.19756/j.issn.0253-3820.261093
乳腺癌3D肿瘤球模型构建及其元素空间分布的同步辐射X射线荧光成像分析
Construction of A 3D Breast Cancer Spheroid Model and Synchrotron X-ray Fluorescence Imaging Analysis of Its Spatial Elemental Distribution
摘要
Abstract
Compared with conventional two-dimensional(2D)adherent cell culture,three-dimensional(3D)tumor spheroids can better recapitulate the spatial architecture and microenvironmental characteristics of tumor tissues,thus providing a more physiologically relevant model for investigating elemental metabolism in cancer cells.In this study,a 3D breast cancer spheroid culture system was established using agarose-coated U-bottom plates,and anin situ analytical method was developed for characterizing the spatial distribution of multiple elements within tumor spheroids by combining this model with synchrotron micro-X-ray fluorescence(micro-XRF)imaging.The results showed that among the three types of tested breast cancer cell lines,namely 4T1,MCF-7,and MDA-MB-231,MDA-MB-231 cells exhibited the best spheroid-forming ability and generated compact spheroids with clear boundaries and controllable sizes.Synchrotron micro-XRF imaging analysis revealed that,compared with 2D-cultured cells,the signals of Ca,Fe,and Zn were significantly enhanced in 3D tumor spheroids and displayed a spatially heterogeneous distribution pattern characterized by peripheral enrichment and relative depletion in the central region.This study established an analytical platform integrating a 3D tumor spheroid model with synchrotron micro-XRF elemental imaging,providing an effective approach for investigating tumor-associated metal element metabolism under conditions that more closely resemble the in vivo microenvironment.关键词
乳腺癌细胞/3D肿瘤球/同步辐射微区X射线荧光/元素/空间分布Key words
Breast cancer cells/3D tumor spheroids/Synchrotron micro-X-ray fluorescence/Elements/Spatial distribution引用本文复制引用
高越,张继超,郭琳洁,李江,诸颖,刘霞..乳腺癌3D肿瘤球模型构建及其元素空间分布的同步辐射X射线荧光成像分析[J].分析化学,2026,54(5):892-900,9.基金项目
国家自然科学基金项目(Nos.32501272,22525605,22325406,32301185)资助. Supported by the National Natural Science Foundation of China(Nos.32501272,22525605,22325406,32301185). （Nos.32501272,22525605,22325406,32301185）
