赣南医科大学学报2026，Vol.46Issue(5)：421-428,8.DOI:10.3969/j.issn.2097-7174.2026.05.003
木犀草素功能性脂质体制备处方工艺优化及其对Hep3B细胞的影响
Optimization of the preparation process for functional liposomes containing luteolin and their effects on Hep3B cells
摘要
Abstract
Objective:To optimize the preparation process of transferrin modified functional liposomes of magnolol and to investigate their effects on Hep3B cells.Methods:Transferrin-modified luteolin-loaded liposomes(TF-LUT@LIP)was synthesized using the thin-film dispersion method.The encapsulation efficiency served as the evaluation criterion.Optimal preparation conditions were determined through single-factor experiments and response surface optimization.The effects on Hep3B cell viability and uptake were assessed via CCK-8 assays and cellular uptake experiments.Results:The optimal formulation preparation conditions are as follows:bile-to-lipid ratio of 1∶6.906,drug-to-lipid ratio of 1∶21.267,ultrasonication power of 38%(total power 650 W),and hydration temperature of 50℃.The response surface methodology-optimized luteolin liposomes achieved an average encapsulation efficiency of 96.94%.When the target molecule loading was 3%,the prepared TF-LUT@LIP exhibited(95.31±0.03)%,an average particle size of(184.53±0.61)nm,a zeta potential of(-23.800±0.500)mV,and excellent stability.TF-LUT@LIP exhibited stronger concentration-dependent inhibitory effects on Hep3B cells than free luteolin(LUT).Compared to non-targeted liposomes,TF-LUT@LIP demonstrated significantly enhanced uptake behavior.Conclusion:TF-LUT@LIP prepared via the optimal synthesis process exhibits high encapsulation efficiency,uniform morphology,and consistent particle size distribution.It demonstrates significant inhibitory effects on Hep3B cells and exhibits favorable cellular uptake behavior.关键词
木犀草素/转铁蛋白/脂质体/响应面优化/Hep3B细胞Key words
Luteolin/Transferrin/Liposomes/Response surface optimization/Hep3B cells分类
医药卫生引用本文复制引用
娄思思,李风叶,杨海龙..木犀草素功能性脂质体制备处方工艺优化及其对Hep3B细胞的影响[J].赣南医科大学学报,2026,46(5):421-428,8.基金项目
江西省国际科技合作项目(20232BBH80011) （20232BBH80011）
赣州市科技计划项目(2024RC05) （2024RC05）
