赣南医科大学学报2026，Vol.46Issue(5)：429-432,4.DOI:10.3969/j.issn.2097-7174.2026.05.004
免疫荧光染色法在下呼吸道真菌感染诊断中的临床价值
The clinical value of immunofluorescence staining in the diagnosis of lower respiratory tract fungal infections
摘要
Abstract
Objective:To evaluate the clinical value of immunofluorescence staining in the diagnosis of lower respiratory tract fungal infections.Methods:Data of 2 224 inpatients with clinically confirmed pulmonary fungal infections in the First Affiliated Hospital of Gannan Medical University between January 1,2020,and December 31,2024 were selected.The detection results of alveolar lavage fluid by immunofluorescence staining and Gram staining were retrospectively analyzed.The detection rate,accuracy,sensitivity,specificity,positive predictive value,and negative predictive value of the two detection methods were calculated respectively.Results:A total of 2 224 alveolar lavage fluid samples were obtained,among which 911 samples were stained by immunofluorescence staining,with a detection rate of 25.47%,accuracy of 51.37%,sensitivity of 25.75%,specificity of 74.79%,positive predictive value of 48.28%,and negative predictive value of 52.43%.A total of 1 313 samples were stained by Gram staining,with a detection rate of 4.95%,accuracy of 55.67%,sensitivity of 6.11%,specificity of 95.99%,positive predictive value of 55.38%,and negative predictive value of 55.69%.The detection rate and sensitivity of alveolar lavage fluid samples stained by immunofluorescence staining were higher than those stained by Gram staining,while the accuracy and specificity were not higher than those stained by Gram staining,and the differences were statistically significant(P<0.05).The positive and negative predictive values were lower than those of Gram staining,but there was no significant difference(P>0.05).Conclusion:Immunoflu-orescence staining of alveolar lavage fluid can quickly and accurately identify fungi with a low risk of missed diagnosis,and has important clinical value in the diagnosis of lower respiratory tract fungal infections.关键词
下呼吸道真菌感染/免疫荧光染色法/革兰氏染色法Key words
Lower respiratory tract fungal infection/Immunofluorescence staining method/Gram staining分类
医药卫生引用本文复制引用
刘倩,罗孝青,曾海慧,周建荣..免疫荧光染色法在下呼吸道真菌感染诊断中的临床价值[J].赣南医科大学学报,2026,46(5):429-432,4.基金项目
赣州市科技计划项目(赣市科[2018]50号) （赣市科[2018]50号）
