河北医学2026，Vol.32Issue(5)：713-720,8.DOI:10.3969/j.issn.1006-6233.2026.05.02
LncRNA LAMTOR5-AS1调节miR-873-5p/E2F3信号轴对胃癌细胞恶性生物学行为的影响
Effect of LncRNA LAMTOR5-AS1 on Malignant Biological Behaviors of Gastric Cancer Cells by Regulating the miR-873-5p/E2F3 Signaling Axis
摘要
Abstract
Objective:To discuss the effect of long non-coding RNA(LncRNA)LAMTOR5-AS1 on the malignant biological behaviors of gastric cancer(GC)cells by regulating the microRNA-873-5p(miRNA-873-5p)/E2F transcription factor 3(E2F3)signaling axis.Methods:The expression levels of LncRNA LAMTOR5-AS1,miR-873-5p and E2F3 mRNA in GC cell lines of each group were detected by qRT-PCR.The dual-luciferase reporter gene assay was used to verify the interactions between LncRNA LAMTOR5-AS1 and miR-873-5p,as well as between miR-873-5p and E2F3.AGS cells were randomly assigned into the Ctrl group,sh-NC group,sh-LAMTOR5-AS1 group,sh-LAMTOR5-AS1+anti-NC group,and sh-LAM-TOR5-AS1+anti-miR-873-5p group.The expression of mRNA in cells of each group was detected by qRT-PCR.The proliferation of AGS cells was detected by MTS assay and colony formation assay.The scratch assay was performed to measure the migration of AGS cells.Transwell assay was performed to detect AGS cells inva-sion.The protein expression of MCM2,E2F3,Snail and Bad in AGS cells was measured by Western blot.Results:The expression of LncRNA LAMTOR5-AS1 and E2F3 mRNA in the GC cell line was raised,while the expression of miR-873-5p decreased(P<0.05).Compared with the sh-NC group and the Ctrl group,the sh-LAMTOR5-AS1 group showed lower clone number,OD490 value,scratch healing rate,invasion num-ber,the expression of LncRNA LAMTOR5-AS1,E2F3(both mRNA and protein),MCM2,and Snail,while the apoptosis rate,miR-873-5p and Bad were notably higher(P<0.05).Compared with the sh-LAMTOR5-AS1 group and the sh-LAMTOR5-AS1+anti-NC group,the sh-LAMTOR5-AS1+anti-miR-873-5p group showed higher clone number,OD490 value,scratch healing rate,invasion number,E2F3(both mRNA and protein),MCM2,and Snail,while the apoptosis rate,miR-873-5p and Bad were notably lower(P<0.05).LncRNA LAMTOR5-AS1 was able to target and negatively regulate miR-873-5p,and miR-873-5p was able to target and negatively regulate E2F3.Conclusion:Knockdown of LncRNA LAMTOR5-AS1 can inhibit the malignant biological behaviors of GC cells by regulating the miR-873-5p/E2F3 signaling axis.关键词
胃癌/长链非编码 RNA/LAMTOR5-AS1/微小 RNA-873-5p/E2F 转录因子3/细胞恶性生物学行为Key words
Gastric cancer/Long non-coding RNA/LAMTOR5-AS1/MicroRNA-873-5p/E2F transcription factor 3/Cell malignant biological behaviors引用本文复制引用
陈碧薇,侯倩倩,郑岳林,李辰..LncRNA LAMTOR5-AS1调节miR-873-5p/E2F3信号轴对胃癌细胞恶性生物学行为的影响[J].河北医学,2026,32(5):713-720,8.基金项目
河北省医学科学研究课题计划项目(编号:20261160) （编号:20261160）
