河北医学2026，Vol.32Issue(5)：720-728,9.DOI:10.3969/j.issn.1006-6233.2026.05.03
LncRNA PVT1调控miR-9-5p/HIPK2轴对缺氧复氧诱导的心肌细胞损伤的影响
The Effect of LncRNA PVT1 on Hypoxia/reoxygenation-Induced Cardiomyocyte Injury by Regulating the miR-9-5p/HIPK2 Axis
摘要
Abstract
Objective:To discuss the effect of long non-coding RNA(LncRNA)plasmacytoma variant translocation 1(PVT1)on hypoxia/reoxygenation(H/R)-induced cardiomyocyte injury by regulating the microrNA-9-5p(miR-9-5p)/homeodomain-interacting protein kinase 2(HIPK2)axis.Methods:AC16 cells were separated into the CG group,H/R group,sh-NC group,sh-PVT1 group,sh-PVT1+NC-inhibitor group,and sh-PVT1+miR-9-5p-inhibitor group.Except for the CG group,the remaining groups were all treated with H/R:8 hours in a hypoxic environment(94%N2,5%CO2,1%O2)at 37℃,followed by 12 hours of reoxygenation uder normoxic environment(95%O2 and 5%CO2).The expression levels of LncRNA PVT1,miR-9-5p and HIPK2 in AC16 cells were detected by qRT-PCR.The proliferation of AC16 cells was detected by MTS assay and colony formation assay.The apoptosis of AC16 cells was detected by flow cytome-try.The release levels of myocardial cell injury indicators(LDH,CK)in the supernatant of AC16 cells were detected by ELISA.DCFH-DA was used to detect the ROS levels in AC16 cells.The contents of oxidative stress(OS)indicators(SOD,MDA)and inflammatory factors(TNF-α,IL-6)in the lysate of AC16 cells were determined by ELISA.Moreover,the protein expression levels of Ki67,HIPK2,p53 and Cleaved Caspase-3 in AC16 cells were detected by Western blot.Results:LncRNA PVT1 could target and negatively regulate miR-9-5p,and miR-9-5p could target and negatively regulate HIPK2.Compared with the CG group,the H/R group had lower OD490 value,clone number,miR-9-5p,SOD,Ki67 and p53,as well as higher apoptosis rate,average fluorescence intensity of ROS,the LncRNA PVT1,HIPK2 at mRNA and pro-tein levels,LDH,CK,MDA,TNF-α,IL-6,and Cleaved Caspase-3(P<0.05).Compared with the H/R group and sh-NC group,the sh-PVT1 group had higher OD490 value,clone number,miR-9-5p,SOD,Ki67 and p53,as well as lower apoptosis rate,average fluorescence intensity of ROS,the LncRNA PVT1,HIPK2 at mRNA and protein levels,LDH,CK,MDA,TNF-α,IL-6,and Cleaved Caspase-3(P<0.05).Com-pared with the sh-PVT1 group and sh-PVT1+NC-inhibitor group,the sh-PVT1+miR-9-5p-inhibitor group had lower OD490 value,clone number,miR-9-5p,SOD,Ki67 and p53,as well as higher apoptosis rate,av-erage fluorescence intensity of ROS,the LncRNA PVT1,HIPK2 at mRNA and protein levels,LDH,CK,MDA,TNF-α,IL-6,and Cleaved Caspase-3(P<0.05).Conclusion:Knockdown of LncRNA PVT1 may alleviated H/R-induced cardiomyocyte injury by regulating the miR-9-5p/HIPK2 axis.关键词
心肌细胞损伤/长链非编码 RNA/浆细胞瘤变异易位1/微小 RNA-9-5p/同源结构域相互作用蛋白激酶2/缺氧复氧Key words
Cardiomyocyte injury/Long non-coding RNA/Plasmacytoma variant translocation 1/MicroRNA-9-5p/Homeodomain interacting protein kinase 2/Hypoxia/reoxygenation引用本文复制引用
刘芳,刘欣,刘沙沙,解娟..LncRNA PVT1调控miR-9-5p/HIPK2轴对缺氧复氧诱导的心肌细胞损伤的影响[J].河北医学,2026,32(5):720-728,9.基金项目
河北省医学科学研究课题(编号:20261384) （编号:20261384）
