河北医学2026，Vol.32Issue(5)：729-737,9.DOI:10.3969/j.issn.1006-6233.2026.05.04
hsa_circ_0005245调控miR-873-5p/STMN1轴对甲状腺癌生长和转移的作用机制
The Mechanism of hsa_circ_0005245 Regulating miR-873-5p/STMN1 Axis on the Growth and Metastasis of Thyroid Cancer
摘要
Abstract
Objective:To explore the effect of hsa_circ_0005245 on the growth and metastasis of thyroid cancer by regulating the microRNA-873-5p(miR-873-5p)/stathmin 1(STMN1)axis.Methods:QRT-PCR was used to detect the mRNA levels of 0005245,miR-873-5p,and STMN1 in thyroid cancer cell lines(KTC-1,TPC-1,C643)and tissues.A dual-luciferase assay was used to examine the binding interactions between STMN1 and miR-873-5p,and between hsa_circ_0005245 and miR-873-5p;KTC-1 cells were as-signed into Control group,sh-NC group,sh-0005245 group,sh-0005245+anti-NC group,sh-0005245+an-ti-miR-873-5p group,the proliferation,migration,and invasion of KTC-1 cells in each group were detec-ted.Western blot and immunohistochemistry were used to detect the expression of MMP-2,MMP-9,PCNA,epithelial-mesenchymal transition(EMT)markers(N-cadherin,vimentin,E-cadherin)and STMN1 Protein Expression.Results:The mRNA expression of hsa_circ_0005245 and STMN1 increased in thyroid cancer tis-sues and thyroid cancer cell lines(KTC-1,TPC-1,C643 cells),while the expression of miR-873-5p de-creased(P<0.05).The StarBase database showed a binding relationship between hsa_circ_0005245 and miR-873-5p,and between STMN1 and miR-873-5p.Compared with the sh-NC group,the expression of hsa_circ_0005245,STMN1,PCNA,MMP-2,MMP-9,N-Cadherin,and Vimentin decreased in the sh-0005245 group,the expression of miR-873-5p and E-Cadherin increased,and the Edu positivity rate,cell migration number,and invasive cell number decreased(P<0.05).Compared with the sh-0005245+anti-NC group,the expression of miR-873-5p and E-Cadherin decreased in the sh-0005245+anti miR-873-5p group,the expression of PCNA,MMP-2,MMP-9,N-Cadherin,Vimentin,and STMN1 increased,and the Edu positivity rate,cell migration number,and invasive cell number increased(P<0.05).Tumor volume and weight were smaller in the sh-0005245 group than sh-NC group,hsa_circ_0005245 and STMN1 expression was lower,and miR-873-5p expression was higher than sh-NC group(P<0.05).Conclusion:Inhibition of hsa_circ_0005245 expression can target the miR-873-5p/STMN1 axis to suppress biological behaviours in thyroid cancer cells,including proliferation,migration,invasion,and EMT.关键词
甲状腺癌/hsa_circ_0005245/微小 RNA-873-5p/抑微管装配蛋白1轴/生长/转移Key words
Thyroid cancer/hsa_circ_0005245/MicroRNA-873-5p/Stathmin 1 axis/Growth/Transfer引用本文复制引用
陆皓东,郭欣,杜静海,李辉,孙文滨,甄艳..hsa_circ_0005245调控miR-873-5p/STMN1轴对甲状腺癌生长和转移的作用机制[J].河北医学,2026,32(5):729-737,9.基金项目
河北省2023年度医学科学研究课题计划项目(编号:20231772) （编号:20231772）
