河北医学2026，Vol.32Issue(5)：738-744,7.DOI:10.3969/j.issn.1006-6233.2026.05.05
归脾汤通过调控AMPK/mTOR通路改善产后子宫复旧不全的机制研究
A Study on the Mechanism of Gui-Pi Decoction in Improving Incomplete Uterine Involution Postpartum by Regulating the AMPK/mTOR Pathway
摘要
Abstract
Objective:To investigate the therapeutic effect and underlying mechanism of Gui-Pi Decoc-tion(GPD)on postpartum uterine involution deficiency in rats.Methods:A rat model of postpartum uterine involution deficiency was established and randomly divided into model group,GPD-low-dose(4.6g/kg)group,GPD-high-dose(9.3g/kg)group,and GPD-high-dose(9.3g/kg)plus AMPK inhibitor(Com-pound C,10mg/kg)group;normal rats served as the control group(n=10 each).All groups received corre-sponding drugs or saline by gavage/intraperitoneal injection twice daily for 7 consecutive days.Uterine bleed-ing volume was recorded.Serum TNF-α,IL-1β,and IL-6 levels were measured by ELISA.Uterine histopa-thology was evaluated by HE staining;apoptosis was detected by TUNEL.Immunohistochemistry was used to examine uterine expression of Bax,Bcl-2,and cleaved caspase-3.Western blot was used to determine uter-ine AMPK,mTOR,and their phosphorylated proteins.Results:Compared with the control group,the model group exhibited increased bleeding volume and elevated serum TNF-α,IL-1β and IL-6(P<0.05);thick-ened endometrium,distorted glands,disordered myometrium,massive inflammatory infiltration,higher apop-totic rate,up-regulated Bax and cleaved caspase-3,increased p-mTOR/mTOR ratio(P<0.05),and down-regulated Bcl-2 and p-AMPK/AMPK ratio(P<0.05).Compared with the model group,both GPD doses reduced bleeding volume and serum TNF-α,IL-1β and IL-6 levels(P<0.05),attenuated endometrium,and contributed to the well-defined muscle layer and reduced inflammatory cells,diminished apoptosis and Bax/cleaved caspase-3 expression,decreased p-mTOR/mTOR ratio(P<0.05),and elevated Bcl-2 and p-AMPK/AMPK ratio(P<0.05)in a dose-dependent manner;these beneficial effects can be reversed by Compound C(P<0.05).Conclusion:Gui-Pi Decoction promotes postpartum uterine involution in rats by suppressing inflammation and apoptosis,an effect likely mediated through modulation of the AMPK/mTOR pathway.关键词
产后子宫复旧不全/归脾汤/炎症因子/凋 亡/AMPK/mTOR 通路Key words
Postpartum uterine involution deficiency/Gui-Pi decoction/Inflammatory cyto-kines/Apoptosis/AMPK/mTOR pathway引用本文复制引用
刘丽洁,刘晶,褚伟莎,马淑英,谷向向..归脾汤通过调控AMPK/mTOR通路改善产后子宫复旧不全的机制研究[J].河北医学,2026,32(5):738-744,7.基金项目
2024年度中医药类科学研究课题(编号:2024134) （编号:2024134）
