河北医学2026，Vol.32Issue(5)：758-764,7.DOI:10.3969/j.issn.1006-6233.2026.05.08
阳和通脉丸调控HMGB1/TLR4通路改善糖尿病下肢动脉硬化闭塞症大鼠动脉炎症
Yanghe Tongmai Pill Improves Arterial Inflammation in Rats with Diabetic Lower Limb Arteriosclerosis Obliterans by Regulating HMGB1/TLR4 Pathway
摘要
Abstract
Objective:To explore the effect of Yanghe Tongmai pill on arterial inflammation in rats with diabetic lower limb arteriosclerosis obliterans,based on regulating the HMGB1/TLR4 signaling pathway.Methods:SD rats were randomly divided into control group,model group,positive drug beprost sodium group,and low-,medium-,and high-dose Yanghe Tongmai pill groups,with 8 rats in each group.A rat model of diabetic lower extremity arteriosclerosis obliterans was constructed by high-fat and high-sugar diet,streptozotocin injection and femoral artery ligation.HE staining was used to detect pathological changes in ar-terial vascular and tibialis anterior muscle tissues.The levels of nitric oxide(NO),endothelin-1(ET-1),IL-6,IL-1β and TNF-α in serum were detected by ELISA.Immunohistochemical staining was used to detect the level and localization of HMGB1 in arterial vascular tissues.Western blot was used to detect the protein expression of HMGB1,TLR4 and p-NF-κB p65/NF-κB p65 in arterial vascular tissues.Results:Compared with the control group,the arterial vascular and tibialis anterior muscle tissue of the rats in the model group showed obvious pathological damage,and the NO level in serum decreased whereas the levels of ET-1,IL-6,IL-1β,TNF-α increased(P<0.05),and the expression of HMGB1,TLR4,p-NF-κB p65/NF-κB p65 in arterial vascular tissue increased(P<0.05).Compared with the model group,the pathological damage of ar-terial vascular and tibialis anterior muscle tissues reduced in the positive drug group,low-,medium-and high-dose groups of Yanghe Tongmai pills,and the NO level in serum increased whereas the levels of ET-1,IL-6,IL-1β,TNF-α decreased(P<0.05),and the expression of HMGB1,TLR4,p-NF-κB p65/NF-κB p65 in arterial vascular tissue decreased(P<0.05).Moreover,the effects of Yanghe Tongmai pills were dose-dependent.The effect of high-dose Yanghe Tongmai Pill was better than that of positive drug(P<0.05).Conclusion:Yanghe Tongmai pill may alleviate arteriosclerosis obliterans of diabetic lower extremities in rats by inhibiting arterial inflammation,and the mechanism may be related to inhibition of HMGB1/TLR4 signaling pathway.关键词
糖尿病下肢动脉硬化闭塞症/阳和通脉丸/HMGB1/TLR4 信号通路/炎 症/内皮功能Key words
Diabetic lower limb arteriosclerosis obliterans/Yanghe tongmai pill/HMGB1/TLR4 signaling pathway/Inflammation/Endothelial function引用本文复制引用
孔五宝,陈广先,吴进,刘超,牛跃龙..阳和通脉丸调控HMGB1/TLR4通路改善糖尿病下肢动脉硬化闭塞症大鼠动脉炎症[J].河北医学,2026,32(5):758-764,7.基金项目
河北省中医药管理局科研计划项目(编号:2022260) （编号:2022260）
