河南农业大学学报2026，Vol.60Issue(3)：401-412,12.DOI:10.16445/j.cnki.1000-2340.20260413.001
正向遗传学筛选在动物病原真菌基因功能解析与降毒改良中的应用研究进展
Research progress on the application of forward genetic screening in gene function analysis and virulence attenuation of animal fungal pathogens
摘要
Abstract
This review systematically elucidates the mechanisms of action of forward genetics screening methods,including ultraviolet mutagenesis,chemical mutagenesis,Agrobacterium tumefaciens-mediated transformation,and transposon-mediated random insertion mutagenesis,and specifically summarizes their applications in gene function analysis of animal pathogenic fungi,mycotoxin degrada-tion,and strain improvement.By comparing the advantages and limitations of different techniques,this review reveals the current shortcomings in mutagenesis efficiency,controllability of mutation loci,high-throughput screening systems,as well as the coverage and representativeness of mutant libraries.Future research should focus on developing efficient and targeted mutagenesis techniques,precise screening strategies,and deepend the integration of multi-omics technologies in the phenotypic analy-sis of mutants.These researches will advance the systematic elucidation of gene functions,enhance the efficient degradation of mycotoxins,and provide robust technical foundation for controlling animal fungal diseases and optimizing industrial and agricultural fungal strains.关键词
正向遗传学筛选/动物病原真菌/毒素降解/基因功能/菌株改良Key words
forward genetics screening/animal pathogenic fungi/mycotoxin degradation/gene func-tion/strain improvement分类
农业科技引用本文复制引用
孟瑶,贺赞婷,楚天欣,赵福杰,赵友宝,张晶..正向遗传学筛选在动物病原真菌基因功能解析与降毒改良中的应用研究进展[J].河南农业大学学报,2026,60(3):401-412,12.基金项目
国家自然科学基金青年基金项目(32402947) （32402947）
河南农业大学杰出人才项目(30500946) （30500946）
