化学试剂2026，Vol.48Issue(6)：1-10,10.DOI:10.13822/j.cnki.hxsj.2026.0094
UHPLC-HRMS结合AntDAS剖析不同产地金银花特征物质及产地溯源研究
Analysis of Characteristic Components and Geographical Origin Traceability of Lonicerae Japonicae Flos from Different Producing Areas by UHPLC-HRMS Combined with AntDAS
摘要
Abstract
The chemical compositions of Lonicerae japonicae Flos samples from four producing areas—Fengqiu,Henan;Xinmi,Henan;Pingyi,Shandong and Zhongwei,Ningxia—were analyzed,and a geographic origin traceability model was established to provide technical support for the authentic identification of Lonicerae japonicae Flos and the standardized development of the Lonicerae japonicae Flos industry.Ultrahigh-performance liquid chromatography-high-resolution mass spectrometry(UHPLC-HRMS)and the data analysis software AntDAS-DDA were used for compound data extraction,alignment,screening of differential components,and compound identification.Geographic origin discrimination models constructed using four classical chemometric methods were evaluated based on the screened differential metabolites.A total of 62 differential metabolites belonging to 11 categories,including flavonoids,coumarins,and phenolic acids,were screened and identified.The partial least-squares discriminant analysis model,constructed based on the differential metabolites,exhibited excellent goodness of fit and predictive ability(R2>0.9,Q2>0.8)and accurately distinguished Lonicerae japonicae Flos samples from the four producing areas,with a model accuracy of 98.60%.Significant differences were found in differential metabolites of Lonicerae japonicae Flos from different producing regions.Lonicerae japonicae Flos from Xinmi area had higher flavonoid contents,while Fengqiu-derived samples were richer in terpenoids.The established origin discrimination model could effectively differentiate sample origins.These results are expected to facilitate the standardized development of the Loniceras japonicas Flos industry in China.关键词
金银花/产地判别/超高效液相色谱-高分辨质谱联用技术(UHPLC-HRMS)/AntDAS-DDA/化学计量学Key words
Lonicerae japonicae Flos/geographical origin discrimination/UHPLC-HRMS/AntDAS-DDA/chemometrics分类
化学化工引用本文复制引用
白莲,周婕婕,余海花,王龙鹤,李淑芳,刘文斌,樊亚玲,韩璐,于永杰..UHPLC-HRMS结合AntDAS剖析不同产地金银花特征物质及产地溯源研究[J].化学试剂,2026,48(6):1-10,10.基金项目
国家自然科学基金项目(22378214) （22378214）
宁夏自然科学基金项目(2023AAC05038,2023AAC03253) （2023AAC05038,2023AAC03253）
宁夏回族自治区重点研发计划项目(2026BEG02035) （2026BEG02035）
河南省自然科学基金青年科学基金(C类)项目(262300422413) （C类）
河南省农业科学院基本科研业务费专项项目(2026ZC91) （2026ZC91）
宁夏医科大学重点科研项目(XJKF230202,XM2020021) （XJKF230202,XM2020021）
陕西中烟工业有限责任公司重点研发项目(BA000-ZB23004,BA000-ZB25004). （BA000-ZB23004,BA000-ZB25004）
