家畜生态学报2026,Vol.47Issue(5):53-60,8.DOI:10.3969/j.issn.1673-1182.2026.05.008
缓释包被尿素对藏西北绒山羊瘤胃组织形态、蛋白酶活性及微生物多样性的影响
Effects of Slow-released Urea on Rumen Tissue Morphology,Proteinase Activity and Microbial Diversity of Tibetan Cashmere Goats
摘要
Abstract
To investigate the effects of slow-released urea on rumen morphology,proteinase activity and microbial diversity of Tibetan cashmere goats,thirty 15-month-old Tibetan cashmere goats(half male and half female)were divided into 3 groups with 10 replicates per group and 1 sheep per replicate according to single factor level.They were divided into control group,urea group(2%)and slow-released urea group(2.4%).The pre-test period lasted for 7 days and the trial period lasted for 49 days.The results showed as follows:(1)the rumen papillary height and keratin thickness in urea group were lower than those in con-trol group and slow-released urea group(P<0.05).(2)The rumen protease activity and pepsin activity in slow-released urea group were higher than those in control group and urea group(P<0.05).(3)Com-pared with control group and slow-released urea group,the relative abundance of Actinobacteriota,Chlo-roflexi(P<0.01)and Firmicutes(P<0.05)in urea group was increased and Bacteroidota was decreased(P<0.05).The rumen of cashmere goats in the slow-released urea group had higher relative abundance of Patescibacteria than that in the control group and the urea group(P<0.01),and the rumen of cashmere goats in the control group had higher relative abundance of Verrucomicrobiota than that in the urea group and the slow-released urea group(P<0.05).Compared with the control group and the slow-released urea group,the relative abundance of Christensenellaceae_R-7_group in urea group(P<0.01),Firmicutes_un-classified and NK4A214 group(NK4A214_group)were increased(P<0.05),while Rikenellaceae_RC9_gut_group(P<0.01)and Prevotella(P<0.05)were decreased.The rumen of cashmere goats in the urea group had lower relative abundance of bacteria F082 than that in the control group(P<0.05).The relative abundance of Candidatus_Saccharimonas in slow-released urea group was higher than that in control group and urea group(P<0.01).In conclusion,dietary urea supplementation changed rumen microflora structure of cashmere goats.Compared with urea,dietary supplemented with slow-released urea could in-crease rumen protease activity,and has no negative effect on rumen development of cashmere goats.关键词
尿素/缓释/绒山羊/瘤胃组织形态/微生物多样性Key words
urea/slow-release/cashmere goat/rumen tissue morphology/microbial diversity分类
农业科技引用本文复制引用
阿旺措吉,郑琳,仁青措姆,达瓦,旦增曲珍,巴贵,索朗达,吴玉江,德吉..缓释包被尿素对藏西北绒山羊瘤胃组织形态、蛋白酶活性及微生物多样性的影响[J].家畜生态学报,2026,47(5):53-60,8.基金项目
西藏十四五重大科技专项(XZ202101ZD0001N) (XZ202101ZD0001N)
国家现代农业产业技术体系(CARS-39-32) (CARS-39-32)