家畜生态学报2026，Vol.47Issue(5)：70-76,7.DOI:10.3969/j.issn.1673-1182.2026.05.010
捕食线虫性真菌Duddingtonia flagrans DF-shz3株的分离鉴定及其捕食活性与产孢特性研究
Isolation and Identification of The Nematode-Trapping Fungus Duddingtonia flagrans Strain DF-shz3 and Its Predatory Activity and Sporulation Characteristics
摘要
Abstract
This study aimed to isolate a nematode-trapping fungal strain with high predatory activity and strong sporulation capacity,providing a candidate microorganism for the development of biocontrol a-gents against nematode infections in herbivorous animals.Soil samples were collected from the Tianshan Pasture in Xinjiang,China,and screened for nematode-trapping fungi.The isolated strains were identified based on morphological characteristics and molecular phylogenetic analysis.Their predatory efficiency and sporulation traits were also evaluated.A single strain,designated DF-shz3,was successfully isolated.The colony appeared white,with septate and branched hyphae,and produced abundant chlamydospores and a small number of conidia.The chlamydospores were globose with spherical surface ornamentations,while the conidia were pyriform or ovoid with a single septum located centrally.Phylogenetic analysis based on the ITS sequence revealed 100％homology between strain DF-shz3 and Duddingtonia flagrans,clustering with the reference sequence KP257593.1.Both morphological and molecular evidence confirmed that DF-shz3 belongs to Duddingtonia flagrans.Strain DF-shz3 exhibited a high predation rate of 97.93％against third-stage larvae of Caenorhabditis elegans,forming adhesive networks as its trapping structure.When cultured on YPSSA medium at 30℃ for 28 days,DF-shz3 produced the highest yield of chlamydospores.In this study,a strain of the nematode-trapping fungus Duddingtonia flagrans suitable for the prevention and control of nematodiasis in native herbivorous animals in Xinjiang was isolated.关键词
捕食线虫性真菌/Duddingtonia flagrans/分离鉴定/捕食活性/产孢特性Key words
predatory nematode fungi/Duddingtonia flagrans/isolation and identification/predatory activity/sporulation characteristic分类
农业科技引用本文复制引用
聂宇航,乔军,张程志,马喜喜,李杰,魏立翔,孙焱森,李若冰,才学鹏,孟庆玲..捕食线虫性真菌Duddingtonia flagrans DF-shz3株的分离鉴定及其捕食活性与产孢特性研究[J].家畜生态学报,2026,47(5):70-76,7.基金项目
国家自然科学基金(32260888,32060801) （32260888,32060801）
新疆自治区研究生科研创新计划项目(XJGRI2015038) （XJGRI2015038）
国家大学生创新创业训练计划项目(202410759005) （202410759005）
