江西农业学报2026，Vol.38Issue(6)：1-8,8.DOI:10.19386/j.cnki.jxnyxb.2026.06.001
水稻核隐性雄性不育系的创制及其在多系品种培育中的应用
Development of Rice Recessive Genic Male Sterile Lines and Their Application in Multi-line Varieties Breeding
摘要
Abstract
At present,the goal of rice breeding has shifted from one-sided emphasis on yield in the past to comprehensive and balanced development of many traits such as high quality,high yield and multi-resistance.It is an inevitable trend for future breeding to comprehensively utilize the identified favorable genes that control excellent agronomic traits.There are obvious limitations in the traditional technical thinking of cultivating pure-line parents when pyramiding multiple genes controlling favorable traits in rice.In contrast,the development of multi-line varieties can avoid these limitations and is regarded as an important technical strategy to break through the bottleneck of current rice breeding.The application of recessive genic male sterile lines in the backcross breeding of multi-line varieties can greatly improve breeding efficiency and help to break the linkage drag.In this study,the TDR gene was site-specific knocked out in japonica rice using the CRISPR-Cas9 system.Positive transgenic plants were obtained by hygromycin resistance marker screening,and sterile lines free of transgenic components were acquired via sequencing and PCR analysis.A method for the propagation of homozygous male sterile lines,as well as a technical scheme for breeding multi-line varieties using this recessive genic male sterile line were obtained.The generation of novel rice sterile materials by site-specific knockout of the TDR gene using CRISPR-Cas9 technology,together with the matched propagation method for sterile lines and technical scheme established for multi-line variety cultivation,possesses important reference value for the breeding and application of rice multi-line varieties.关键词
水稻/CRISPR-Cas9/核隐性雄性不育系/多系品种Key words
Rice/CRISPR-Cas9/Recessive genic male sterile line/Multi-line varieties分类
农业科技引用本文复制引用
王燕宁,唐秀英,黄永兰,苌兴超,王会民,芦明,龙起樟,万建林..水稻核隐性雄性不育系的创制及其在多系品种培育中的应用[J].江西农业学报,2026,38(6):1-8,8.基金项目
江西省农业科学院基础研究与领军人才培养专项(JXSNKYJCRC202202) （JXSNKYJCRC202202）
江西省农业科学院博士启动项目(2012CBS002) （2012CBS002）
江西省农作物良种联合攻关项目(2022JXNZWZY01). （2022JXNZWZY01）
