棉花学报2026，Vol.38Issue(1)：12-21,10.DOI:10.11963/zrcs20250054
沉默GhMLO6基因可增强棉花对黄萎病的抗性
Silencing the GhMLO6 gene improves cotton Verticillium wilt resistance
摘要
Abstract
[Objective]This study aimed to investigate the function of the GhMLO6 gene in cotton Verticillium wilt resistance and identify a candidate gene for disease resistance breeding in cotton.[Methods]Quantitative real-time polymerase chain reaction(qRT-PCR)was used to analyze the expression pattern of GhMLO6.The virus-induced gene silencing(VIGS)technique was employed to transiently silence GhMLO6 in cotton to study its function in Verticillium wilt resistance.Agrobacterium-mediated transient expression technology was applied to Nicotiana benthamiana to explore the subcellular localization of GhMLO6.[Results]The qRT-PCR results revealed that GhMLO6 presented the highest expression level in cotton roots,followed by stems and leaves.Compared with the control,infection with Verticillium dahliae significantly increased the expression level of GhMLO6 in roots.Compared with the control plants in which empty vectors were used,GhMLO6-silenced plants displayed a significant decrease in disease index and relative fungal DNA content.After stem recovery and cultivation,the accumulation of hyphae was reduced,and the resistance of cotton to Verticillium wilt was significantly increased.Transient expression analysis indicated that GhMLO6 localizes on the cell membrane.[Conclusion]GhMLO6 is localized to the cell membrane and performs a negative regulatory function in cotton Verticillium wilt resistance.Therefore materials with Verticillium wilt resistance could be obtained via gene editing technology.关键词
GhMLO6/棉花/黄萎病/抗病性/负调控Key words
GhMLO6/cotton/Verticillium wilt/disease resistance/negative regulation引用本文复制引用
赵茹,刘廷利,唐宇翔,李小莉,黄梓成,徐文君,张娜,贲爱玲,王立科,陈全战..沉默GhMLO6基因可增强棉花对黄萎病的抗性[J].棉花学报,2026,38(1):12-21,10.基金项目
国家自然科学基金(32372499 （）
32502507) （）
江苏省自然科学基金(BK20221426) （BK20221426）
江苏省高校自然科学基金(25KJD210005) （25KJD210005）
