棉花学报2026，Vol.38Issue(1)：35-49,15.DOI:10.11963/wppcs20250044
基于内参菌株的棉花立枯丝核菌的荧光定量检测方法的建立及应用
Establishment and application of a fluorescent quantitative detection method for Rhizoctonia solani causing cotton seedling damping-off based on an internal reference strain
摘要
Abstract
[Objective]This study aims to establish a rapid quantitative detection method for Rhizoctonia solani,the causal agent of cotton seedling damping-off in soil,and to investigate the correlation between pathogen abundance and disease occurrence,providing a scientific basis for effective disease control.[Methods]A quantitative detection method for R.solani AG-4 HGⅢ in soil was developed using internal reference strain correction and quantitative real-time polymerase chain reaction(qPCR).Pot experiments and field trials were conducted to study the relationship between R.solani abundance in soil and the incidence of cotton seedling damping-off.[Results]A qPCR-based quantitative detection method incorporating internal reference stain correction was successfully established,with high specificity and a sensitivity of 10 copies·μL-1.The critical threshold of R.solani in soil for severe disease occurrence and seedling mortality risk was determined to be 106 copies·g-1.[Conclusion]A rapid quantitative detection method for R.solani in cotton field soil was successfully developed,enabling accurate quantification of the pathogen and the early disease risk warning.关键词
立枯丝核菌/棉花/立枯病/qPCR/风险预警Key words
Rhizoctonia solani/cotton/seedling damping-off/qPCR/risk warning引用本文复制引用
王培培,马平,董丽红,张家齐,李社增,赵卫松,鹿秀云,付一帆,郭志刚,郭庆港..基于内参菌株的棉花立枯丝核菌的荧光定量检测方法的建立及应用[J].棉花学报,2026,38(1):35-49,15.基金项目
河北省农林科学院科技创新专项课题(2026KJCXZX-ZBS-5) （2026KJCXZX-ZBS-5）
国家棉花产业技术体系(CARS-15-19) （CARS-15-19）
河北省自然科学基金(C2025301115) （C2025301115）
河北省省级科技计划(242N6501Z) （242N6501Z）
