南京林业大学学报（自然科学版）2026，Vol.50Issue(3)：26-35,10.DOI:10.12302/j.issn.1000-2006.202402026
盐胁迫下柳树良种组培苗的抗氧化特性及同工酶谱变化
Changes in antioxidant characteristics and isozyme bands in Salix variety plantlets in vitro under salinity stress
摘要
Abstract
[Objective]Studying the response of Salix variety to salt stress and the threshold of salt tolerance could provide reference for further research salt resistance mechanism and expansion of planting scale in saline-alkali land.[Method]The tissue culture plantlets of S.×jiangsuensis'J172'('J172')were treated with NaCl 0,50,100,150,200 and 250 mmol/L for 10-20 days under in vitro culture.The changes of morphology,physiological indexes and antioxidant enzyme isozyme bands in'J172'plantlets were studied.[Result]'J172'plantlets had certain salt tolerance,and its salt tolerance threshold was lower than 150 mmol/L NaCl,its growth was seriously damaged and even death under higher than 200 mmol/L NaCl.Under mild salt stress(50 mmol/L NaCl),the growth of'J 172'plantlets were not affected,and the levels of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),proline and glutathione(GSH)were mildly increased,other indexes were no significant changes.Under moderate salt stress(100-150 mmol/L NaCl),the levels of chlorophyll(Chl)and soluble protein(SP)decreased slightly,while the levels of relative conductivity(REC),malondialdehyde(MDA)or H2O2 began to increase,and the levels of GSH and SOD,POD and CAT reached a high point,respectively,and the salt tolerance reached the highest at these treatment.However,at high salt stress(200-250 mmol/L NaCl),the contents of Chl,SP and GSH decreased sharply and reached the lowest point,while the levels of REC,MDA reached the highest point,respectively,and the levels of GSH and SOD,POD and CAT activities decreased significantly,indicating that'J172'plantlets were subjected to severe oxidative stress.Salt stress also led to the significant enhancement of isoenzyme bands such as CuZnSOD in chloroplasts and cytoplasm,MnSOD in mitochondria as well as POD2,POD3 and POD4.[Conclusion]The'J172'clones had certain salt-resistant ability and could be cultivated in salt-bearing areas lower than 150 mmol/L.关键词
柳树组培苗/盐胁迫/生理响应/同工酶Key words
Salix tissue culture seedlings/salt stress/physiological response/isoenzyme分类
农业科技引用本文复制引用
钟颂正,施士争,王红玲,胡菲,狄晶晶,陈颖..盐胁迫下柳树良种组培苗的抗氧化特性及同工酶谱变化[J].南京林业大学学报（自然科学版）,2026,50(3):26-35,10.基金项目
江苏省农业科技自主创新资金项目(CX(19)100401). （CX(19）
