南京林业大学学报（自然科学版）2026，Vol.50Issue(3)：45-53,9.DOI:10.12302/j.issn.1000-2006.202412028
氮沉降背景下模拟硫酸型酸雨酸度对杉木幼苗根系生理特征的影响
Effects of simulated sulfuric acid rain on root physiological characteristics of Chinese fir seedlings under the condition of nitrogen deposition
摘要
Abstract
[Objective]This study aims to elucidate the effects of sulfuric acid rain acidity on the physiological characteristics of Cunninghamia lanceolata(Chinese fir)seedling roots under nitrogen deposition,providing a theoretical basis for the sustainable management of Chinese fir plantations in acid-nitrogen deposition area.[Method]A pot experiment was conducted using one-year-old Chinese fir seedlings to simulate different levels of nitrogen deposition(no nitrogen addition(N0),low nitrogen addition at 5 g/(m2·a)(N1),and high nitrogen addition at 15 g/(m2·a)(N2))and acid rain(no acid addition(pH 7.0,S0),weak acid addition(pH 4.5,S1),and strong acid addition(pH 2.5,S2)).The experiment investigated the effects of these treatments on seedling root physiology and soil nutrient dynamics.[Result]Under no nitrogen treatment(N0),decreasing pH of sulfuric acid rain significantly reduced root activity and soluble protein content,while increasing root malondialdehyde(MDA)content,peroxidase(POD)and catalase(CAT)activities,and root biomass.Similarly,under no acid rain treatment(S0),nitrogen addition significantly decreased soluble protein content,while root biomass increased with higher nitrogen addition.Low nitrogen addition(N1)had no significant effect on root activity,MDA content,or CAT activity,whereas high nitrogen addition(N2)significantly reduced root activity and increased MDA content and CAT activity.Under N1 and N2 treatments,root activity and soluble protein content were significantly lower in weak acid(S1)than in strong acid(S2)treatments.In the N1 treatment,sulfuric acid rain significantly increased root MDA content,POD,and superoxide dismutase(SOD)activities.Under the N2 treatment,decreasing pH of sulfuric acid rain gradually reduced root MDA content and SOD activity,while POD activity initially decreased and then increased.Additionally,both sulfuric acid rain and nitrogen addition reduced soil pH,with nitrogen addition significantly lowering soil pH under weak acid treatment but showing no significant effect under strong acid treatment.High nitrogen addition alone increased soil nitrate and ammonium nitrogen content(NO3--N and NH4-N).In the low nitrogen(N1)treatment,weak acid(S1)resulted in the highest soil NO3--N content,significantly higher than strong acid(S2).Under high nitrogen(N2)treatment,sulfuric acid rain significantly reduced both nitrate and ammonium nitrogen content.In the no nitrogen(N0)treatment,sulfuric acid rain significantly decreased soil NO3--N and available phosphorus content.[Conclusion]Sulfuric acid rain and nitrogen deposition exhibited significant interactive effects on the physiological characteristics of Chinese fir roots.Although the intensity of sulfuric acid rain in China has gradually decreased,the synergistic effect of mild acid rain and nitrogen deposition may still exacerbate soil acidification,reversing the beneficial impact of low nitrogen deposition on root physiology,reducing seedling root activity,and hindering the sustainable management of Chinese fir plantations.关键词
硫酸型酸雨/氮沉降/杉木/根系活力/土壤养分Key words
sulfuric acid rain/nitrogen deposition/Cunninghamia lanceolata(Chinese fir)/root activity/soil nutrients分类
农业科技引用本文复制引用
周悦湘,王宇浩,张金池,蔡祺,廖艺,薛仁贵,刘鑫..氮沉降背景下模拟硫酸型酸雨酸度对杉木幼苗根系生理特征的影响[J].南京林业大学学报（自然科学版）,2026,50(3):45-53,9.基金项目
江苏省自然科学基金青年基金项目(BK20200785) （BK20200785）
江苏省研究生科研与实践创新计划项目(KYCX23_1240) （KYCX23_1240）
国家自然科学基金项目(32271844) （32271844）
江苏省科技计划项目(BE2022420). （BE2022420）
