南京林业大学学报（自然科学版）2026，Vol.50Issue(3)：54-61,8.DOI:10.12302/j.issn.1000-2006.202501023
不同浓度氮素对东京野茉莉苗木质素合成的影响
The effect of different nitrogen concentrations on lignin biosynthesis in Styrax tonkinensis seedlings
摘要
Abstract
[Objective]This study investigated the effects of different nitrogen(N)fertilizer concentrations on lignin content and the activities of key enzymes involved in lignin biosynthesis in the stems of Styrax tonkinensis seedlings.This study aims to provide a theoretical and practical basis for improving the papermaking quality ofS.tonkinensis wood through nitrogen management.[Method]Three-year-oldS.tonkinensis seedlings were subjected to five N application concentrations:0(CK),1.5(N1),3.0(N2),6.0(N3),and 9.0(N4)g per plant.Fertilizer was applied on the afternoons of June 15,July 5,and July 25.Stem samples were collected on June 15(pre-treatment),July 15,August 15,and September 15 to determine lignin content,lignin monomer composition(G,S,H types),and the activities of key lignin biosynthesis enzymes.[Result]The lignin content in the stems showed an initial decrease followed by an increase over time.On July 15 and August 15,the N1 treatment significantly increased lignin content,whereas the N2,N3 and N4 treatments resulted in lower content than that of the CK and maintained consistently low levels.The contents of G-and S-type lignin monomers generally increased then decreased with rising N concentration,while H-type monomer content showed no clear trend.The S/G ratio was higher under the N1 and N2 treatments on July 15.Within a certain range,low N concentrations enhanced the activities of phenylalanine ammonia-lyase(PAL),peroxidase(POD),cinnamic acid dehydrogenase(CAD),and trans-cinnamic acid-4-monooxygenase(C4H),whereas high concentrations suppressed them.Correlation analysis revealed a significant positive correlation between lignin content and POD activity on July 15(P＜0.05),and negative correlations with CAD,C4H and PAL activities.During the last three sampling dates,POD activity remained positively or highly significantly positively correlated with lignin content.On July 15 and August 15,the S/G ratio showed a significant negative correlation with G-type monomers and a significant positive correlation with S-type monomers.[Conclusion]Increased nitrogen application during the fast-growing stage of S.tonkinensis reduce the activities of key lignin biosynthesis enzymes and decrease lignin synthesis and accumulation.Applying N at the N2 concentration(3.0 g/plant)in the early fast-growing stage reduces lignin content of stems while increasing the S/G ratio,thereby improving papermaking performance.关键词
氮素/东京野茉莉/木质素合成/木质素单体/酶活性/造纸性能Key words
nitrogen/Styrax tonkinensis/lignin synthesis/lignin monomer/enzyme activity/papermaking performance分类
农业科技引用本文复制引用
苏欣怡,陈俊娜,彭辉武,陈娟,黄卫和,喻方圆..不同浓度氮素对东京野茉莉苗木质素合成的影响[J].南京林业大学学报（自然科学版）,2026,50(3):54-61,8.基金项目
南京市产学研合作后补助项目(201722081) （201722081）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
江西省省级林业补助专项项目(创新专项[2021]16号). （创新专项[2021]16号）
