日用化学工业（中英文）2026，Vol.56Issue(5)：614-622,9.DOI:10.3969/j.issn.2097-2806.2026.05.008
浙江红山茶籽油酶解产物抗D-gal诱导皮肤成纤维细胞衰老作用及机制研究
Anti-aging effects and mechanism of enzymatic hydrolysate of Camellia chekiangoleosa Hu seed oil against D-galactose-induced senescence in human skin fibroblasts
卢琦 1向文叠 1李广涛 1尹世鹏 1高宏旗 1余果1
作者信息
- 1. 林清轩&上海交通大学细胞级抗衰联合研究实验室,上海林清轩生物科技股份有限公司,上海 201612
- 折叠
摘要
Abstract
This study investigated the protective effects and underlying mechanisms of the enzymatic hydrolysate from Camellia chekiangoleosa Hu seed oil(EHCO)on D-galactose(D-gal)-induced senescence in human normal foreskin fibroblasts(HFF).A cellular senescence model was established by treating HFFs with 60 mg/mL D-gal.Cells were co-treated with D-gal and EHCO(0.1%or 0.5%)for 24 hours,and cell viability and senescence levels were subsequently assessed.The content of ATP and the activity of SOD were measured using ELISA kits.Mitochondrial function and proliferative capacity were evaluated via fluorescent staining for ROS,JC-1,and EDU.The mRNA expression levels of senescence-associated and inflammation-related genes were quantified by RT-qPCR.Furthermore,the protein expression of apoptosis-related and autophagy-related markers,along with the phosphorylation status of P53 and ERK,were analyzed by Western blotting.In EHCO-treated HFF,the mRNA levels of P16,P21,P53,IL-1β,and IL-6,as well as the phosphorylation levels of P53 and ERK,significantly reduce in a dose-dependent manner(P<0.05).Concurrently,the pro-apoptotic BAX/BCL-2 protein ratio decreases,while the expression of the autophagy marker protein LC3B elevates.This study demonstrates that EHCO,obtained through lipase-catalyzed hydrolysis of Camellia chekiangoleosa Hu,promotes cell proliferation and protects against D-gal-induced senescence in HFF.Mechanistically,EHCO exerts anti-skin-aging effects by improving fibroblast function via the suppression of the ERK-P53 signaling axis and the activation of cellular autophagy.关键词
浙江红山茶籽油酶解产物/成纤维细胞/细胞自噬/衰老Key words
enzymatic hydrolysate of Camellia chekiangoleosa Hu seed oil/fibroblasts/autophagy/senescence分类
化学化工引用本文复制引用
卢琦,向文叠,李广涛,尹世鹏,高宏旗,余果..浙江红山茶籽油酶解产物抗D-gal诱导皮肤成纤维细胞衰老作用及机制研究[J].日用化学工业（中英文）,2026,56(5):614-622,9.
