山东医药2026，Vol.66Issue(5)：30-33,4.DOI:10.3969/j.issn.1002-266X.2026.05.006
电针穴位刺激对2型糖尿病大鼠糖代谢及骨骼肌内质网应激的影响
Effects of electroacupuncture at acupoints on glucose metabolism and endoplasmic reticulum stress in skeletal muscle of type 2 diabetic rats
摘要
Abstract
Objective To observe the effects of electroacupuncture at acupoints on glucose metabolism and endoplas-mic reticulum stress(ERS)in the skeletal muscle of rats with type 2 diabetes mellitus(T2DM),and to explore the poten-tial mechanism underlying its improvement of glucose metabolism.Methods According to the random number table meth-od,18 rats were divided into the blank group,model group,and electroacupuncture group,with 6 rats in each group.Rats in the blank group were fed with a normal diet.Rats in the model group and electroacupuncture group were fed with a high-fat and high-sugar diet and intraperitoneally injected with 1%streptozotocin(STZ)solution to establish T2DM mod-els.Rats in the electroacupuncture group received electroacupuncture intervention once a day,6 times a week for 6 weeks.Rats in the blank group and model group were only grasped and fixed for 20 minutes without intervention.The glu-cose metabolism indicators,including fasting blood glucose(FBG),serum fasting insulin(FINS),and homeostasis model assessment of insulin resistance(HOMA-IR),were compared among the groups.The ultrastructure of endoplasmic reticu-lum in skeletal muscle cells was observed using atomic force microscopy(AFM).The expression levels of endoplasmic re-ticulum stress-related proteins glucose-regulated protein 78(GRP78),activating transcription factor 6(ATF6),protein ty-rosine phosphatase 1B(PTP1B),and insulin receptor substrate 1(IRS1)in the skeletal muscle tissues were detected by Western blotting.Results Compared with the blank group,the model group showed significantly higher FBG,serum FINS,and HOMA-IR after modeling and intervention(all P<0.05),with a reticular tubular structure of the endoplasmic reticulum,higher protein expression levels of GRP78,ATF6,and PTP1B,and lower IRS1 protein expression in skeletal muscle tissues(all P<0.05).Compared with the model group,the electroacupuncture group showed significantly lower FBG,serum FINS,and HOMA-IR after intervention(all P<0.05),with normalized endoplasmic reticulum morphology,lower protein expression levels of GRP78,ATF6,and PTP1B,and higher IRS1 protein expression in skeletal muscle tis-sue(all P<0.05).Conclusion Electroacupuncture may improve glucose metabolism in T2DM rats by regulating the ex-pression of ERS-related proteins,inhibiting ERS in skeletal muscle,and up-regulating IRS1 protein expression.关键词
2型糖尿病/电针/内质网应激/骨骼肌/糖代谢/大鼠Key words
type 2 diabetes mellitus/electroacupuncture/endoplasmic reticulum stress/skeletal muscle/glucose metabolism/rats分类
医药卫生引用本文复制引用
刘珂,李瑞,张莉,李晓璐,林嘉宜,刘尚瑾,芮芳芳..电针穴位刺激对2型糖尿病大鼠糖代谢及骨骼肌内质网应激的影响[J].山东医药,2026,66(5):30-33,4.基金项目
国家自然科学基金面上项目(82474652). （82474652）
