陕西中医2026，Vol.47Issue(6)：736-740,747,6.DOI:10.3969/j.issn.1000-7369.2026.06.003
益肾固胎颗粒通过调控Th17/Treg细胞平衡改善复发性流产的作用机制研究
Mechanistic study of Yishen Gutai granules in improving recurrent spontaneous abortion by regulating Th17/Treg cell balance
摘要
Abstract
Objective:To investigate the mechanism by which Yishen Gutai granules improve pregnancy out-comes in mice with recurrent spontaneous abortion(RSA)by regulating the Th17/Treg cell balance.Methods:Thirty CBA/J female mice were randomly divided into five groups:the control group,the normal pregnancy group,the RSA model group,the Yishen Gutai granules group,and the cyclosporine A group.From day 0 to day 14 of gestation,mice in the Yishen Gutai granules and cyclosporine A groups were administered 3.6 g/kg and 25 mg/kg,respectively,by gavage.After the final administration,placental pathology was observed via hematoxylin-eosin(HE)staining.Serum levels of interleukin-6(IL-6),interleukin-17α(IL-17α),transforming growth factor-β(TGF-β),and interleukin-10(IL-10)were detected by enzyme-linked immunosorbent assay(ELISA).The proportion of Th17 and Treg cells in peripheral blood was analyzed by flow cytometry.Results:Compared with the RSA model group,placental pathologi-cal injury in the Yishen Gutai granules group was significantly improved,with markedly alleviated inflammatory cell infiltration and tissue swelling.Serum levels of IL-6 and IL-17α decreased by 42.7％and 56.9％,respectively(P＜0.01),while expression levels of TGF-β and IL-10 increased by 2.1-fold and 1.7-fold,respectively.The proportion of Th17 cells decreased from(7.5±0.8)％to(3.2±0.5)％,the proportion of Treg cells increased from(8.2±0.9)％to(12.7±1.1)％,and the Th17/Treg ratio was restored from 0.32 to 0.25(P＜0.01).Conclusion:Yishen Gutai granules may improve pregnancy outcomes in RSA mice by regulating the Th17/Treg cell balance and enhan-cing immune tolerance at the maternal-fetal interface.These findings provide a molecular immunological basis for the traditional Chinese medicine method of"tonifying the kidney to secure the fetus".关键词
益肾固胎颗粒/复发性流产/母-胎免疫耐受/Th17/Treg平衡/细胞因子/补肾安胎Key words
Yishen Gutai granules/Recurrent spontaneous abortion/Maternal-fetal immune tolerance/Th17/Treg balance/Cytokines/Tonifying the kidney to secure the fetus分类
医药卫生引用本文复制引用
万茜茜,方熙星,王宋瑶,邵梦秋,KHUANNAPHA Phanthavong,王仁容,刘旭,姜丽娟..益肾固胎颗粒通过调控Th17/Treg细胞平衡改善复发性流产的作用机制研究[J].陕西中医,2026,47(6):736-740,747,6.基金项目
国家自然科学基金地区科学基金资助项目(82460950) （82460950）
云南省科技厅中医联合专项青年项目(202401AZ070001-112) （202401AZ070001-112）
云南中医药大学院校联合基金资助一般项目(XYLH2024026) （XYLH2024026）
