生物技术通报2026，Vol.42Issue(5)：5-15,11.DOI:10.13560/j.cnki.biotech.bull.1985.2026-0429
基于高分辨率质谱的微生物宏代谢组解析方法研究进展
Progress in High-resolution Mass Spectrometry-based Approaches for Microbial Meta-metabolomics
摘要
Abstract
Elucidating the regulatory mechanisms and ecological functions of microbial metabolism is crucial for understanding the carbon biogeochemical cycling,predicting carbon-climate feedbacks,and modulating carbon flows in engineered applications.Microbial meta-metabolomics conceptualizes organic molecules as a"molecular community",enabling a holistic investigation into the composition and dynamics of metabolites at the community level.High-resolution mass spectrometry has emerged as a powerful approach for large-scale profiling of organic metabolites,and the resulting molecular fingerprints can be deeply explored by leveraging ecological analytical frameworks.However,challenges remain in linking metabolites to microbial functions and attributing metabolic processes.This review systematically summarizes recent methodological advances in meta-metabolomics,including the extended application of ecological mechanisms,the construction of molecular ecological networks,and multi-dimensional molecular profiling,while also highlighting current technical limitations.Looking forward,integrating multi-omics spatiotemporal analyses,identifying key metabolic roles,and extending ecological frameworks will be critical for establishing this field as a frontier in understanding ecosystem functions and carbon regulation.关键词
宏代谢组/生态过程/分子生态网络/分子特征Key words
meta-metabolomics/ecological processes/molecular ecological network/molecular feature引用本文复制引用
杨兴盛,李春格,胡秋龙,邓晔,伍绍龙,冯凯,王尚,彭玺,赵博,刘明倩,顾松松,何晴..基于高分辨率质谱的微生物宏代谢组解析方法研究进展[J].生物技术通报,2026,42(5):5-15,11.基金项目
国家科技重大专项"深地复杂地质环境与微生物适应条件"(2024ZD1000601),中国烟草总公司重点研发项目(110202402009) （2024ZD1000601）
