陕西医学杂志2026，Vol.55Issue(6)：748-753,760,7.DOI:10.3969/j.issn.1000-7377.2026.06.005
普鲁卡因调节MAPK/ERK信号通路对甲状腺癌B-CPAP细胞增殖、迁移、侵袭及凋亡的影响实验研究
Impacts of procaine on proliferation,migration,invasion and apoptosis of thyroid cancer B-CPAP cells by regulating MAPK/ERK pathway
摘要
Abstract
Objective:To explore the impacts of procaine(PCA)on the proliferation,migration,invasion and ap-optosis of thyroid cancer B-CPAP cells by regulating the mitogen-activated protein kinase(MAPK)/extracellular regulated protein kinase(ERK)pathway.Methods:Human thyroid cancer cell B-CPAP was cultured and assigned in-to control group(CK group),low-dose PCA group(L-PCA group),medium-dose PCA group(M-PCA group),high-dose PCA group(H-PCA group),and the H-PCA+MAPK activator anisomycin group(H-PCA+Anisomycin group).Cloning formation experiment was used to detect the proliferation of B-CPAP cells.Transwell assay was im-plemented to detect migration and invasion of B-CPAP cells.Acridine orange(AO)/ethidium bromide(EB)staining was implemented to detect apoptosis of B-CPAP cells.Western blot was implemented to detect proliferation,migra-tion,invasion,apoptosis,and MAPK/ERK pathway-related protein expression.Nude mouse transplantation tumor experiment(control group and PCA group,8 nude mice in each group)was used to detect the volume and weight of transplanted tumors.Immunohistochemical staining was implemented to detect the expression of Cyclin D1,Ki-67,Vimentin,epithelial cadherin(E-cadherin),Cysteine aspartic acid specific protease-3(Caspase-3),B-cell lymphoma-2(Bcl-2),and Bcl-2 associated X protein(Bax).Results:Compared with the CK group,the L-,M-,and H-PCA groups had a decrease in the number of clones formed,the number of migrating cells,the number of invading cells,and the expression of Cyclin D1,Ki-67,Vimentin,Bcl-2 proteins,and the ratios of p-P38 MAPK/P38 MAPK and p-ERK1/2/ERK1/2,and had an increase in apoptosis rate,and the expression of E-cadherin,Caspase-3,and Bax proteins(all P＜0.05).Compared with the H-PCA group,the H-PCA+anisomycin group had an increase in the number of clones formed,the number of migrating cells,the number of invading cells,and the expression of Cyclin D1,Ki-67,Vimen-tin,Bcl-2 proteins,and the ratios of p-P38 MAPK/P38 MAPK and p-ERK1/2/ERK1/2,and had a decrease in apop-tosis rate,the expression of E-cadherin,Caspase-3,and Bax proteins(all P＜0.05).Compared with the control group,the PCA group had a decrease in volume and weight of the transplanted tumors,and Cyclin D1,Ki-67,Vimen-tin,and Bcl-2 positive cell rates,and an increase in E-cadherin,Caspase-3,and Bax positive cell rates(all P＜0.05).Conclusion:The inhibitory effect of PCA on proliferation,migration and invasion as well as the pro-apoptotic effect on thyroid cancer B-CPAP cells may be achieved by inhibiting the MAPK/ERK pathway.关键词
甲状腺癌/普鲁卡因/丝裂原活化蛋白激酶/细胞外信号调节激酶通路/增殖/迁移/侵袭/凋亡Key words
Thyroid cancer/Procaine/Mitogen-activated protein kinase/extracellular signal-regulated kinase pathway/Proliferation/Migration/Invasion/Apoptosis分类
医药卫生引用本文复制引用
何概易南,苏元元,顾新华..普鲁卡因调节MAPK/ERK信号通路对甲状腺癌B-CPAP细胞增殖、迁移、侵袭及凋亡的影响实验研究[J].陕西医学杂志,2026,55(6):748-753,760,7.基金项目
江苏省基础研究计划(自然科学基金)专项资金资助项目(BK20230108) （自然科学基金）
