陕西医学杂志2026，Vol.55Issue(6)：754-760,7.DOI:10.3969/j.issn.1000-7377.2026.06.006
基于Hippo-YAP/TAZ途径探讨丹参酮ⅡA对肝硬化大鼠肝纤维化的改善作用实验研究
Improvement effect of Danshenide ⅡA on liver fibrosis in liver cirrhosis rats based on Hippo-YAP/TAZ pathway
摘要
Abstract
Objective:To investigate the effect of tanshinone Ⅱ A(Tan Ⅱ A)on liver fibrosis in rats with liver cirrhosis,and to analyze its potential mechanism based on the Hippo-YAP/TAZ pathway.Methods:A liver cirrhosis rat model was established by intraperitoneal injection of 40％carbon tetrachloride in peanut oil suspension.The suc-cessfully modeled rats were randomly divided into the model group,the Tan Ⅱ A-L group(intragastric administra-tion of 5 mg/kg Tan Ⅱ A),the Tan Ⅱ A-M group(intragastric administration of 10 mg/kg Tan Ⅱ A),and the TanⅡ A-H group(intragastric administration of 20 mg/kg Tan Ⅱ A),with 15 rats in each group.Another 15 SD rats were randomly selected as the control group.The intervention lasted for 8 weeks.The ELISA kits were used to detect the liver function-related indicators(ALT,AST,and TBIL)in the rat serum.HE staining and Masson staining were used to observe the pathological changes of the rat liver tissue.Immunohistochemical staining was used to detect the protein expression of liver fibrosis-related proteins(TGF-β1,α-SMA).Immunofluorescence was used to detect the expression intensity of α-SMA in HSC.Western blot was used to detect the expression of Hippo-YAP/TAZ pathway-related proteins in liver tissue.Results:Compared with the control group,the liver tissue damage in the model group was more severe,and the expression levels of serum ALT,AST,and TBIL,the expression intensity of α-SMA,and the protein levels of α-SMA,TGF-β1,p-YAP,p-TAZ,and CTGF in liver tissues were significantly increased(all P＜0.05).Compared with the model group,the liver tissue damage in the Tan Ⅱ A-L group,Tan Ⅱ A-M group,and Tan Ⅱ A-H group was alleviated,and the expression levels of serum ALT,AST,and TBIL,the expression intensity of α-SMA,and the protein levels of α-SMA,TGF-β1,p-YAP,p-TAZ,and CTGF in liver tissues were significantly de-creased(all P＜0.05).Among them,the overall effect of the Tan Ⅱ A-H group was the most significant.Conclusion:Tan Ⅱ A can effectively improve the degree of liver fibrosis in rats,inhibit the activation of HSCs,and its mechanism of action may be related to the inhibition of the Hippo-YAP/TAZ signaling pathway.关键词
肝硬化/肝纤维化/丹参酮ⅡA/肝星状细胞/Yes相关蛋白/含PDZ结合基序的转录共激活因子/大鼠Key words
Liver cirrhosis/Liver fibrosis/Tanshinone ⅡA/Hepatic stellate cells/Yes-associated protein/Transcriptional coactivator with PDZ-binding motif/Rats分类
医药卫生引用本文复制引用
全养雅,张彩英..基于Hippo-YAP/TAZ途径探讨丹参酮ⅡA对肝硬化大鼠肝纤维化的改善作用实验研究[J].陕西医学杂志,2026,55(6):754-760,7.基金项目
湖南省自然科学基金资助项目(2024JJ51192) （2024JJ51192）
