陕西医学杂志2026，Vol.55Issue(6)：769-774,6.DOI:10.3969/j.issn.1000-7377.2026.06.008
麦冬皂苷B调节cGAS-STING信号通路对甲状腺癌TPC-1细胞凋亡、增殖和免疫逃逸的影响
Effects of OP-B on apoptosis,proliferation and immune escape of thyroid cancer TPC-1 cells by regulating cGAS-STING signaling pathway
摘要
Abstract
Objective:To investigate the effect of Ophiopogonin B(OP-B)on the apoptosis,proliferation and im-mune escape of thyroid cancer TPC-1 cells and the regulatory mechanism of cyclic GMP-AMP synthase(cGAS)-stimulator of interferon gene(STING)signaling pathway.Methods:TPC-1 cells were grouped into TPC-1 group(un-treated TPC-1 cells),L-OP-B group(2.5 μmol/L OP-B treated TPC-1 cells),M-OP-B group(5 μmol/L OP-B trea-ted TPC-1 cells),H-OP-B group(10 μmol/L OP-B treated TPC-1 cells),RU.521 group(100 μmol/L cGAS-STING signaling pathway inhibitor RU.521 treated TPC-1 cells),and H-OP-B+RU.521 group(100 μmol/L RU.521 and 10 μmol/L OP-B co-treated TPC-1 cells).MTT method was applied to detect the toxicity of OP-B on normal human thyroid cell line NTHY ORI 3-1 cells and TPC-1 cells.MTT method was applied to detect TPC-1 cell proliferation.Flow cytometry was applied to detect apoptosis of TPC-1 cells.CD8+T cells were co-cultured with TPC-1 cells in each group,detection of CD8+T cell viability was performed by trypan blue staining and flow cytometry.ELISA method was applied to detect the levels of IFN-γ and TNF-α in the supernatant.Western blot was applied to detect programmed death ligand 1(PD-L1)and cGAS-STING pathway protein levels.Results:Compared with the TPC-1 group,the OD value and PD-L1 protein level in the L-OP-B group,M-OP-B group,and H-OP-B group were obviously reduced,the apoptosis rate of TPC-1 cells,proportion of CD8+T cells,survival rate of CD8+T cells,the IFN-γ and TNF-α levels,cGAS and STING protein levels were obviously increased,and the effect of OP-B showed a dose-de-pendent relationship,while the RU.521 group showed an opposite trend in the above indicators(all P＜0.05).Com-pared with the H-OP-B group,the OD value and protein level of PD-L1 in the H-OP-B+RU.521 group were obvi-ously increased,the apoptosis rate of TPC-1 cells,proportion of CD8+T cells,survival rate of CD8+T cells,the IFN-γand TNF-α levels,cGAS and STING protein levels were obviously reduced(all P＜0.05).Conclusion:OP-B can in-duce apoptosis of TPC-1 cells,inhibit cell proliferation and immune escape,and the mechanism may be related to the activation of cGAS-STING signaling pathway.关键词
甲状腺癌/麦冬皂苷B/增殖/凋亡/免疫逃逸/环状GMP-AMP合成酶-干扰素基因刺激蛋白信号通路Key words
Thyroid cancer/Ophiopogonin B/Proliferation/Apoptosis/Immune escape/Cyclic GMP-AMP syn-thase-stimulator of interferon genes signaling pathway分类
医药卫生引用本文复制引用
陈攀,张煜,赵启生..麦冬皂苷B调节cGAS-STING信号通路对甲状腺癌TPC-1细胞凋亡、增殖和免疫逃逸的影响[J].陕西医学杂志,2026,55(6):769-774,6.基金项目
湖北省卫生健康科研指导性项目(WJ2021F087) （WJ2021F087）
