陕西医学杂志2026，Vol.55Issue(6)：775-780,6.DOI:10.3969/j.issn.1000-7377.2026.06.009
槲皮素通过促进M2型巨噬细胞极化改善膝骨性关节炎大鼠滑膜炎症实验研究
Quercetin improves synovial inflammation in KOA rats by promoting M2-type macrophage polarization
摘要
Abstract
Objective:To investigate the effects and mechanism of quercetin on synovial inflammation in rats with knee osteoarthritis(KOA).Methods:A rat model of KOA was established and randomly divided into the fol-lowing groups(n=10 each):sham-operated group,model group,low-dose quercetin group(50 mg/kg),high-dose quercetin group(100 mg/kg),and celecoxib group(20 mg/kg).All groups received daily intragastric administration of the respective agents for 4 weeks.Behavioral assessments of joint pain were conducted.Histopathological altera-tions in joint tissues were visualized via HE staining.Interleukin-6(IL-6),monocyte chemoattractant protein-1(MCP-1),and IL-10 concentrations in serum and synovial fluid were quantified by ELISA.Synovial protein expres-sion was profiled using immunohistochemistry and Western blot.Specifically,immunohistochemistry was utilized for the detection of tumor necrosis factor-α(TNF-α)and cyclooxygenase-2(COX-2),whereas Western blot was used for the quantification of inducible nitric oxide synthase(iNOS),CD68,arginase1(Arg1),and CD206.Results:Compared with the sham group,KO A model rats exhibited decreased mechanical and thermal pain thresholds(all P＜0.05);IL-6 and MCP-1 levels were elevated and IL-10 level was reduced in serum and synovial fluid(all P＜0.05);cartilage degeneration was accompanied by synovial inflammatory hyperplasia;synovial expression of TNF-α,COX-2,iNOS,and CD68 proteins were increased,along with decreased Arg1 and CD206 proteins(all P＜0.05).Relative to the model group,both low-and high-dose quercetin groups and celecoxib group reversed these changes:mechanical and thermal pain thresholds were increased(all P＜0.05));IL-6 and MCP-1 levels in serum and synovial fluid were de-creased while IL-10 level was increased(all P＜0.05);histopathological damage was alleviated;and TNF-α,COX-2,iNOS,and CD68 protein levels in synovial were reduced,whereas Arg1 and CD206 protein levels were elevated(all P＜0.05).Conclusion:Quercetin alleviates joint pain and attenuates synovial inflammation in KOA rats,possibly by promoting M2 macrophage polarization.关键词
膝骨性关节炎/槲皮素/M2型巨噬细胞极化/滑膜炎症/大鼠Key words
Knee osteoarthritis/Quercetin/M2 macrophage polarization/Synovial inflammation/Rat分类
医药卫生引用本文复制引用
彭潇,伍星,陈宏,何瀚威,邓尚斌,朱毅琳..槲皮素通过促进M2型巨噬细胞极化改善膝骨性关节炎大鼠滑膜炎症实验研究[J].陕西医学杂志,2026,55(6):775-780,6.基金项目
湖南省卫生健康委科研项目(W20243270) （W20243270）
