摘要
Abstract
This study aimed to investigate the mechanisms of continuous cropping obstacles affecting Astragalus membranaceus root metabolism and evaluated the mitigation effects of exogenous organic materials on such disturbances.A pot experiment was conducted with three treatments:conventional planting,continuous cropping,and continuous cropping+bio-organic fertilizer.The activities of root metabolic enzymes,contents of organic acids,and antioxidant substances were measured.Continuous cropping significantly reduced the activities of sucrase and peroxidase in Astragalus membranaceus roots,decreased the contents of oxalic acid,citric acid,and malic acid,increased the content of malondialdehyde,and reduced the contents of soluble protein and proline.The bio-organic fertilizer treatment significantly improved various metabolic indicators in the roots of Astragalus membranaceus under continuous cropping.Continuous cropping disturbance inhibits the activities of root metabolic enzymes in Astragalus membranaceus,it reduces the secretion capacity of organic acids,and weakens the function of the root antioxidant defense system.The application of bio-organic fertilizer can effectively mitigate the negative effects of continuous cropping disturbance on the root metabolism of Astragalus membranaceus.关键词
黄芪/连作障碍/根系代谢/酶活性/有机酸/抗氧化系统Key words
Astragalus membranaceus/continuous cropping disturbance/root metabolism/enzyme activity/organic acids/anti-oxidant system