西北农业学报2026，Vol.35Issue(6)：1000-1011,12.DOI:10.7606/j.issn.1004-1389.2026.06.002
微生物菌剂对不同品种小麦产量、品质及土壤微生物群落的影响
Effects of Microbial Inoculants on Yield and Quality of Different Wheat Varieties and Soil Microbial Community
摘要
Abstract
To clarify the regulatory effects of microbial inoculants on wheat yield,quality,and soil microorganisms,this study used varieties'Yannong 999'and'Huaimai 43'as experimental materials and established three treatments:control without inoculant(Z0),inoculation with Azospirillum(Z1),and inoculation with Bacillus subtilis(Z2).Field experiments were conducted to systematically determine wheat yield and its components,grain quality indicators,soil physicochemical properties,soil enzyme activities,and soil bacterial community structure using 16S rRNA gene high-throughput sequencing.The results showed that inoculation with Azospirillum and Bacillus subtilis enhanced wheat yield and quality,with the Z2 treatment producing the most pronounced effects.Compared with Z0,the Z2 increased the average yield of the two wheat varieties by 745 kg·hm-2,an increase of 16.14%,Z2 also increased protein and wet gluten contents in YN999 by 18.91%and 17.89%,re-spectively,and in HM43 by 18.42%and 5.82%,respectively.The Z1 treatment resulted in the high-est alkali-hydrolyzable nitrogen content and urease activity,with average increases of 7.23%and 11.32%,respectively,and the enhancement was greater in'Huaimai 43'than in'Yannong 999'.The Z2 treatment led to the highest available phosphorus and potassium contents,as well as sucrose enzyme and catalase activities,with average increases of 26.64%,32.61%,7.06%,and 11.34%,re-spectively,and the improvements were more evident in HM43.16S rRNA sequencing results revealed that,compared with Z0,both Z1 and Z2 significantly increased the Chao1 and Shannon indices.Z1 significantly increased the relative abundances of Proteobacteria and Bacteroidetes,whereas Z2 signifi-cantly increased those of Chloroflexi and Acidobacteria.Correlation analysis indicated that the rela-tive abundance of Acidobacteria was significantly positively correlated with available phosphorus con-tent(P<0.05),while available potassium and available phosphorus contents were significantly posi-tively correlated with yield and quality(P<0.05).In conclusion,the Z2 treatment demonstrates the optimal effects,primarily by increasing the relative abundances of Acidobacteria and Chloroflexi,enhancing urease and sucrose enzyme activities,and improving available phosphorus and available po-tassium contents,thereby significantly enhancing wheat yield and quality.关键词
微生物菌剂/产量/品质/土壤养分/土壤酶活性/微生物群落多样性Key words
Microbial inoculum/Yield/Quality/Soil nutrients/Soil enzyme activity/Microbial com-munity diversity引用本文复制引用
潘洋,郑甲成,任兰天,马东旭,宁佳瑶,邵庆勤..微生物菌剂对不同品种小麦产量、品质及土壤微生物群落的影响[J].西北农业学报,2026,35(6):1000-1011,12.基金项目
国家重点研发计划项目(2022YFD2301402) （2022YFD2301402）
安徽省高校协同创新项目(GXXT-2023-102、GXXT-2023-103) （GXXT-2023-102、GXXT-2023-103）
安徽省重点研发计划项目(2023n06020012) （2023n06020012）
安徽科技学院重点研究项目(2021zrzd09) （2021zrzd09）
凤阳县科技计划项目(2024NY-03). National Key R&D Program of China(No.2022YFD2301402) （2024NY-03）
Anhui Provincial University Collaborative Innovation Project(No.GXXT-2023-102,No.GXXT-2023-103) （No.GXXT-2023-102,No.GXXT-2023-103）
Key R&D Program of Anhui Province(No.2023N06020012) （No.2023N06020012）
Key Research Project of Anhui University of Sci-ence and Technology(No.2021ZRZD09) （No.2021ZRZD09）
Fengyang County Science and Technology Program(No.2024NY-03). （No.2024NY-03）
