刑事技术2026，Vol.51Issue(3)：266-271,6.DOI:10.16467/j.1008-3650.2025.0026
一步法mRNA-PCR荧光复合扩增检测体系在法医案件中的验证
Application of the One-step mRNA-PCR Fluorescence Multiplex Amplification Detection System in Forensic Cases
摘要
Abstract
To evaluate the application potential of the one-step mRNA-PCR fluorescence multiplex amplification detection technology for identifying body fluid stains in case samples,a total of 89 samples suspected to be blood,saliva,semen,and vaginal secretions were collected.These samples were analyzed collaboratively with two forensic DNA laboratories lacking prior RNA operation experience.The laboratories performed total RNA extraction,one-step RT-PCR amplification,and capillary electrophoresis detection using different experimental instruments.The results demonstrated that three housekeeping genes(GAPDH,ACTB,and PRL19)were successfully detected in all 89 case samples.Additionally,specific markers for each body fluid were identified:HBB and HBA for peripheral blood;MMP7 and MMP10 for menstrual blood;STATH and HTN3 for saliva;PRM2 and SEMG1 for semen;and CYP2B7P1 and HBD1 for vaginal fluid.Notably,each participating DNA laboratory was able to independently complete the detection of RNA markers in the case samples,and parallel testing of the same samples across different laboratories yielded consistent results.This study provides robust evidence supporting the application of the one-step mRNA-PCR fluorescence multiplex amplification detection system for the source identification of body fluid stains in forensic case samples.关键词
法医遗传学/体液斑迹鉴定/一步法mRNA-PCR荧光复合扩增检测/外周血/月经血/精液/唾液/阴道分泌物Key words
forensic genetics/body fluids identification/one-step mRNA-PCR/peripheral blood/menstrual blood/semen/saliva/vaginal discharge分类
社会科学引用本文复制引用
赵丽,季安全,赵一霞,宋金平,高峰,李爱强,张艳霞,王项华,胡胜,孙启凡..一步法mRNA-PCR荧光复合扩增检测体系在法医案件中的验证[J].刑事技术,2026,51(3):266-271,6.基金项目
国家重点研发计划(2022YFC3341002) （2022YFC3341002）
中央级公益性科研院所基本科研业务费专项资金项目(2023JB019) （2023JB019）
现场物证溯源技术国家工程实验室开放课题(2020NELKFKT04) （2020NELKFKT04）
