眼科新进展2026，Vol.46Issue(6)：432-436,5.DOI:10.13389/j.cnki.rao.2026.0077
双丹明目胶囊含药血清对高糖诱导的大鼠视网膜神经节细胞损伤的改善作用及其机制
Alleviating effect of medicated serum of Shuangdan Mingmu Capsules on high glucose-induced injury in rat retinal ganglion cells and its mechanism
摘要
Abstract
Objective To investigate the mechanism by which the medicated serum of Shuangdan Mingmu Capsules alleviates high glucose-induced injury in rat retinal ganglion cells(RGCs).Methods Rat RGCs cultured in vitro were di-vided into the blank serum control group(5.5 mmol·L-1 glucose+10％volume fraction of blank serum),medicated serum control group(5.5 mmol·L-1 glucose+10％volume fraction of medicated serum of Shuangdan Mingmu Capsules),blank serum high glucose group(35.0 mmol·L-1 glucose+10％volume fraction of blank serum),and medicated serum treat-ment group(35.0 mmol·L-1 glucose+10％volume fraction of medicated serum of Shuangdan Mingmu Capsules).After 48 hours of intervention,cell viability was assessed using the CCK-8 assay,and the apoptosis rate was measured using flow cytometry.Western blot was used to detect the expression levels of endoplasmic reticulum stress-related proteins,including activating transcription factor 6(ATF6),protein kinase RNA-like endoplasmic reticulum kinase(PERK),C/EBP homolo-gous protein(CHOP),and the phosphorylation level of the eukaryotic translation initiation factor 2 alpha(eIF2α).Results Compared with the blank serum control group and the medicated serum control group,the blank serum high glucose group exhibited a significantly decreased cell viability and a significantly increased apoptosis rate(all P＜0.01).Compared with the blank serum high glucose group,the medicated serum treatment group showed a significantly increased cell viabili-ty and a significantly decreased apoptosis rate(both P＜0.01).Compared with the blank serum control group and the medi-cated serum control group,the expression levels of ATF6,PERK,CHOP,and p-eIF2α in RGCs significantly increased in the blank serum high glucose group(all P＜0.05).Compared with the blank serum high glucose group,the expression levels of the above proteins significantly decreased in the medicated serum treatment group(all P＜0.05).No statistically significant difference was observed in the expression level of eIF2α among the groups(all P＞0.05).Conclusion The medicated serum of Shuangdan Mingmu Capsules significantly alleviates high glucose-induced RGC damage and apoptosis in rats,which may be closely related to its role in regulating the PERK/CHOP/eIF2α signaling pathway and reducing the level of en-doplasmic reticulum stress in cells.关键词
双丹明目胶囊/糖尿病视网膜病变/内质网应激/细胞凋亡Key words
Shuangdan Mingmu Capsules/diabetic retinopathy/endoplasmic reticulum stress/apoptosis分类
医药卫生引用本文复制引用
刘峥嵘,杨婷,赵永旺,秦裕辉..双丹明目胶囊含药血清对高糖诱导的大鼠视网膜神经节细胞损伤的改善作用及其机制[J].眼科新进展,2026,46(6):432-436,5.基金项目
国家自然科学基金项目(编号:81874493) （编号:81874493）
湖南省自然科学基金项目(编号:2022JJ40237) （编号:2022JJ40237）
