眼科新进展2026，Vol.46Issue(6)：437-442,6.DOI:10.13389/j.cnki.rao.2026.0078
YAP1通过结合TEAD2调控靶基因BIRC5参与人晶状体上皮细胞上皮-间质转化
摘要
Abstract
Objective To explore the involvement of Yes-associated protein 1(YAP1)in the epithelial-mesenchymal transition(EMT)of human lens epithelial cells(LECs)by binding to transcriptional enhancer domain transcription factor 2(TEAD2)to regulate the target gene baculovirus inhibitor of apoptosis protein 5(BIRC5).Methods HLE-B3 cells were divided into the Vector group,OE-YAP1 group,and OE-TEAD2 group,and transfected with pCDNA3.1-Vector,pCDNA3.1-YAP1,and pCDNA3.1-TEAD2,respectively.The transfection was observed under an inverted fluorescence microscope.After successful transfection,the cultured HLE-B3 cells were further cultured for 24 hours using DEME with 10 μg·L-1 transfor-ming growth factor-β2 and divided into the Vector+T empty vector group,OE-YAP1+T transfection group,OE-TEAD2+T transfection group,and OE-YAP1+OE-TEAD2+T co-transfection group.Real-time fluorescence quantitative PCR and West-ern blot were used to detect the relative mRNA and protein expression levels of YAP1,TEAD2,BIRC5,E-cadherin,α-smooth muscle actin(α-SMA),and Vimentin in each group of cells.Wild-type vector pGL3-basic-BIRC5-WT containing binding sites and mutant vector pGL3-basic-BIRC5-MUT containing mutation sites were constructed and transfected with pCDNA3.1-TED2 or pCDNA3.1-Vector into HLE-B3 cells.The interaction between TEAD2 and BIRC5 was verified using the dual lucif-erase reporter gene assay.Results The overexpression plasmids YAP1 and TEAD2,as well as the empty vector,were highly expressed in cells in the Vector group,OE-YAP1 group,and OE-TEAD2 group,and can be used for subsequent experi-ments.The relative protein and mRNA expression levels of YAP1,TEAD2,and BIRC5 in the OE-YAP1+OE-TEAD2+T co-transfection group were higher than those in the Vector+T empty vector group and the OE-YAP1+T transfection group,and the differences were statistically significant(all P＜0.05).Compared with the Vector+T empty vector group and the OE-YAP1+T transfection group,the OE-YAP1+OE-TEAD2+T co-transfection group had lower protein and mRNA expression levels of E-cadherin and higher protein and mRNA expression levels of α-SMA and Vimentin,with statistically significant differences(all P＜0.05).The results of the dual luciferase reporter gene assay confirmed that TEAD2 could activate the transcription of BIRC5.Conclusion YAP1 can regulate the target gene BIRC5 to participate in the EMT of LECs by bind-ing to TEAD2,providing a new approach for the prevention and treatment of posterior capsular opacification.关键词
人晶状体上皮细胞/上皮-间质转分化/转录增强结构域转录因子2/杆状病毒凋亡抑制蛋白5/Hippo/Yes相关蛋白信号通路Key words
human lens epithelial cells/epithelial-mesenchymal transition/transcriptional enhancer domain transcrip-tion factor 2/baculovirus inhibitor of apoptosis protein 5/Hippo/Yes-associated protein signaling pathway分类
医药卫生引用本文复制引用
黄建环,郑柳,蒋姣姣,苏星宇,杨彬彬,李金清,陆素青,丁芝祥..YAP1通过结合TEAD2调控靶基因BIRC5参与人晶状体上皮细胞上皮-间质转化[J].眼科新进展,2026,46(6):437-442,6.基金项目
国家自然科学基金项目(编号:8216040180) （编号:8216040180）
桂林市科学研究与技术开发计划项目[编号:市科(2024)17号(20230121-7)] （2024）
