眼科新进展2026，Vol.46Issue(6)：450-453,472,5.DOI:10.13389/j.cnki.rao.2026.0080
青蒿琥酯通过IL-23/IL-17信号通路对实验性自身免疫性葡萄膜炎大鼠模型的干预作用
Interventional effect of artesunate on the rat model of experimental autoim-mune uveitis via the interleukin-23/interleukin-17 signaling pathway
摘要
Abstract
Objective To investigate the interventional effect of artesunate(ART)on rat models of experimental au-toimmune uveitis(EAU)via the interleukin(IL)-23/IL-17 signaling pathway.Methods Totally 48 female Lewis rats were randomly divided into the normal control group,EAU model group,low-concentration ART group,medium-concentration ART group,high-concentration ART group,and triamcinolone acetonide(TA)group.Except for the normal control group,EAU was induced in all other groups.Rats in each ART concentration group received intravitreal injection of corresponding concentrations of ART 2 days before model establishment;rats in the TA group received intravitreal TA injection at the same time point,while the normal control group received no treatment.Anterior chamber inflammatory changes were observed on days 1,4,7,14 and 21 post-induction.Histopathological examination was performed via hematoxylin-eosin staining on days 14 and 21 post-induction.Western blot was used to detect the protein expression levels of IL-23,IL-17 and IL-12 in retinal tissues of rats in each group.Results Ocular inflammation scores:on days 1,4,7,14 and 21 post-induction,scores in the EAU model group were significantly higher than those in the normal control group,while scores in the high-concentration ART group and TA group were significantly lower than those in the EAU model group(all P＜0.05).No statistically significant differences were found between the low-concentration and medium-concentration ART groups and the EAU model group(all P＞0.05).Retinal histopathological section scores:on days 14 and 21 post-induction,scores in the EAU group were sig-nificantly higher than those in the normal control group.On day 14 post-induction,scores in the high-concentration ART group were significantly lower than those in the EAU model group(all P＜0.05).Compared with the normal control group,the relative protein expression levels of IL-23,IL-17 and IL-12 in the EAU model group significantly increased on days 14 and 21 post-induction(all P＜0.05).Compared with the EAU model group,these levels markedly decreased in the high-concen-tration ART group and TA group on day 14 post-induction(all P＜0.05).On day 21 post-induction,TA intervention signifi-cantly reduced the expression of the three cytokines compared with the EAU model group(all P＜0.05).Conclusion In-tervention with 500 g·L-1 ART can significantly alleviate inflammatory responses in the EAU model and markedly reduce the expression of IL-23,IL-17 and IL-12,thereby inhibiting the activation of the IL-23/IL-17 signaling pathway.These findings suggest that the anti-inflammatory mechanism of ART may be associated with the IL-23/IL-17 signaling pathway.关键词
青蒿琥酯/实验性自身免疫性葡萄膜炎/白细胞介素-23/白细胞介素-17/白细胞介素-12Key words
artesunate/experimental autoimmune uveitis/interleukin-23/interleukin-17/interleukin-12分类
医药卫生引用本文复制引用
刘晓娟,郝晓凤,赵伟伟,杜改萍..青蒿琥酯通过IL-23/IL-17信号通路对实验性自身免疫性葡萄膜炎大鼠模型的干预作用[J].眼科新进展,2026,46(6):450-453,472,5.基金项目
青岛市医药卫生科研计划项目(编号:2024-WJKY22) （编号:2024-WJKY22）
