云南民族大学学报（自然科学版）2026，Vol.35Issue(3)：300-308,9.DOI:10.3969/j.issn.1672-8513.2026.03.002
黄芪甲苷调控Nrf2/Keap1信号通路抗脓毒症急性肺损伤效应机制的研究
Study on the mechanism of the anti-sepsis acute lung injury effect of Astragaloside Ⅳ by modulating Nrf2/Keap1 signaling pathway
摘要
Abstract
This study aimed to investigate the mechanism of astragaloside Ⅳ(AS-Ⅳ)in improving sepsis-induced acute lung injury.A model of sepsis-induced acute lung injury was established by cecal ligation and puncture(CLP).After drug intervention,the oxidative stress index and Nrf2/Keap1 signaling pathway-related protein and mRNA expression were detected.The results showed that AS-Ⅳ drug intervention improved the survival rate of septic mice,improved the degree of pathological injury of lung tissue,reduced the level of inflammatory factors,reduced ROS,MDA,and MPO oxidative stress indicators,and increased SOD activity.AS-Ⅳ intervention significantly increased the protein and mRNA expressions of Nrf2 and HO-1.The Nrf2 inhibitor ML385 could significantly reverse the improvement in survival rate and the alleviation of lung pathological injury induced by AS-Ⅳ in mice,and significantly inhibit the increase in SOD activity induced by AS-Ⅳ.ML385 intervention reduced AS-Ⅳ-induced Nrf2 and HO-1 protein expression and mRNA levels.AS-Ⅳ can significantly improve acute lung injury induced by sepsis and inhibit oxidative stress response.Its mechanism may be related to the activation of the Nrf2/Keap1 signaling pathway.关键词
黄芪甲苷/脓毒症/急性肺损伤/Nrf2/Keap1信号通路Key words
astragaloside Ⅳ/sepsis/acute lung injury/Nrf2/Keap1 signaling pathway分类
医药卫生引用本文复制引用
莫庆艳,王敏,巨鸣谦,张春菲,张浩洪,刘帅,黄茂葵,邹楠婷,李晓红,万春平..黄芪甲苷调控Nrf2/Keap1信号通路抗脓毒症急性肺损伤效应机制的研究[J].云南民族大学学报（自然科学版）,2026,35(3):300-308,9.基金项目
国家自然科学基金(81960745) （81960745）
云南省呼吸系统疾病临床医学研究中心开放课题(202102AA100057). （202102AA100057）
